Alexa Fluor 635
Alexa Fluor™ 635 (Ex-Max 633 nm/Em-Max 647 nm)
Alexa Fluor™ 635 (AF635, Alexa 635) has an excitation peak at 633nm and an emission peak at 647nm. It can be used with the following analysis platforms: Flow Cytometry, Immunoassay, IHC, Western Blot.
Excitation & emission spectra
Key facts
- Excitation maximum
- 633 nm
- Emission maximum
- 647 nm
- Optimal laser line
- 628-640
- Optimal filter
- 630/69
- Relative brightness
- 3 of 5
- Extinction coefficient
- 140000
- Commercial antibodies
- 3 from 2 suppliers
Alexa Fluor 635 has an excitation maximum at 633 nm and an emission maximum at 647 nm, a Stokes shift of 14 nm. Its extinction coefficient is 140000 M⁻¹cm⁻¹. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is Alexa Fluor 635?
- Alexa Fluor™ 635 (AF635, Alexa 635) has an excitation peak at 633nm and an emission peak at 647nm. It can be used with the following analysis platforms: Flow Cytometry, Immunoassay, IHC, Western Blot.
- What laser excites Alexa Fluor 635?
- Alexa Fluor 635 is optimally excited by the 628-640 laser line. Its excitation maximum is 633 nm.
- What filter should I use to detect Alexa Fluor 635?
- A 630/69 filter captures the peak of the Alexa Fluor 635 emission near 647 nm with minimal spillover.
- How bright is Alexa Fluor 635?
- Alexa Fluor 635 has a relative brightness of 3 out of 5.
- How many antibodies are available conjugated to Alexa Fluor 635?
- FluoroFinder tracks 3 commercially available Alexa Fluor 635 conjugates, searchable by target, clone, host and application.