MitoView 633
MitoView 633 (Ex-Max 622 nm/Em-Max 648 nm)
Biotium's MitoView dyes are used for live cell mitochondrial staining. These dyes are bright, photostable, non-toxic dyes, and do not require a wash step before Fluorescence Microscopy. While these dyes should be used for live cell staining only, the exception is MitoView Green which can be used for staining fixed cells. MitoView 633 has an excitation peak at 622 nm and an emission peak at 648 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 622 nm
- Emission maximum
- 648 nm
- Optimal laser line
- 628-640
- Optimal filter
- 660/20
- Commercial antibodies
- 2 from 1 supplier
MitoView 633 has an excitation maximum at 622 nm and an emission maximum at 648 nm, a Stokes shift of 26 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is MitoView 633?
- Biotium's MitoView dyes are used for live cell mitochondrial staining. These dyes are bright, photostable, non-toxic dyes, and do not require a wash step before Fluorescence Microscopy. While these dyes should be used for live cell staining only, the exception is MitoView Green which can be used for staining fixed cells. MitoView 633 has an excitation peak at 622 nm and an emission peak at 648 nm.
- What laser excites MitoView 633?
- MitoView 633 is optimally excited by the 628-640 laser line. Its excitation maximum is 622 nm.
- What filter should I use to detect MitoView 633?
- A 660/20 filter captures the peak of the MitoView 633 emission near 648 nm with minimal spillover.
- How many antibodies are available conjugated to MitoView 633?
- FluoroFinder tracks 2 commercially available MitoView 633 conjugates, searchable by target, clone, host and application.