About Brilliant Violet 650

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Brilliant Violet™ 650 (BV650) is a red-emitting tandem fluorophore that can be excited by the 405 nm violet laser and collected using a 660/20 bandpass filter. BV650 has an excitation peak at 405 nm and an emission peak at 645 nm. Due to its unique stoke’s shift, not many dyes have identical excitation and emission spectrums, but the nearest alternatives include SuperBright 645, Qdot™ 655 and eFluor™ 650NC (ThermoFisher). BV650 exhibits a medium level of brightness and is most often used in flow cytometry, specifically, it is an alternative to nanocrystals used for intracellular flow. This dye is part of the Brilliant Violet dye line. The Brilliant Violet dye line itself is very popular because other dyes in the line also use the Brilliant Violet 421 core as the donor molecule, while varied acceptor molecules emit light at various points across the visible light spectrum. This allows for multiple dyes to be compatible while excited by the single laser. In cytometry, a user may populate all the available channels off their 405-violet laser with Brilliant Violet dyes.

Dyes similar to Brilliant Violet 650 based on emission

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7-AAD Alexa Fluor 633 Alexa Fluor 635 ATTO Rho14 BDY 630-X SE BODIPY 630/650 CellTracker Deep Red CF620R CF633 CytoTrack Red DY-481XL E2-Crimson eFluor 650NC eVolve 655 Helix NP NIR Janelia Fluor 635 LysoBrite NIR MagDot 640 MITO-ID (Extracellular O2) MitoView 633 mNeptune mPlum MultiDot 640 Nuclear-ID Red PE-Fire 640 PhenoVue Nile Red Lipid Stain SNARF 1 pH9.0 STAR 635P StarBright Violet 670 SuperBright 645 Syto 61 Sytox AADvanced TagRFP657 Vybrant DyeCycle Orange