About Brilliant Violet 650

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Brilliant™ Violet 650 (BV650) is a red-emitting tandem fluorophore that can be excited by the 405 nm Violet laser and collected using a 660/20 bandpass filter. BV650 has an excitation peak at 405 nm and an emission peak at 645 nm. Due to its unique Stokes shift, not many dyes have identical excitation and emission spectrums, but the nearest alternatives include SuperBright 645, Qdot® 655 and eFluor™ 650NC (ThermoFisher). BV650 exhibits a medium level of brightness and is most often used in flow cytometry, specifically, it is an alternative to nanocrystals used for intracellular flow. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dye from Beckman Coulter and StarBright dyes from Bio-Rad.

Alternative Dyes to Brilliant Violet 650

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SuperBright 645 StarBright Violet 670 Qdot 655

Dyes similar to Brilliant Violet 650 based on emission

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7-AAD Alexa Fluor 633 Alexa Fluor 635 ATTO Rho14 BDY 630-X SE BODIPY 630/650 CellTracker Deep Red CF620R CF633 CytoTrack Red E2-Crimson eFluor 650NC eVolve 655 Helix NP NIR Janelia Fluor 635 LysoBrite NIR MagDot 640 MITO-ID (Extracellular O2) MitoView 633 mNeptune mPlum MultiDot 640 Nuclear-ID Red PE-Fire 640 PhenoVue Nile Red Lipid Stain SNARF 1 pH9.0 STAR 635P SuperBright 645 Syto 61 Sytox AADvanced TagRFP657