LysoBrite NIR
LysoBrite NIR (Ex-Max 635 nm/Em-Max 650 nm)
LysoBrite NIR from AAT Bioquest is a cell-permeant alkaline probe that localizes to acidic compartments inside the cell, such as endosomes and lysosomes. These types of probes are designed for live-cell imaging and do not typically survive fixation and permeabilization. LysoBrite NIR has an excitation peak at 635 nm and an emission peak at 650 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 635 nm
- Emission maximum
- 650 nm
- Optimal laser line
- 628-640
- Optimal filter
- 660/20
LysoBrite NIR has an excitation maximum at 635 nm and an emission maximum at 650 nm, a Stokes shift of 15 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is LysoBrite NIR?
- LysoBrite NIR from AAT Bioquest is a cell-permeant alkaline probe that localizes to acidic compartments inside the cell, such as endosomes and lysosomes. These types of probes are designed for live-cell imaging and do not typically survive fixation and permeabilization. LysoBrite NIR has an excitation peak at 635 nm and an emission peak at 650 nm.
- What laser excites LysoBrite NIR?
- LysoBrite NIR is optimally excited by the 628-640 laser line. Its excitation maximum is 635 nm.
- What filter should I use to detect LysoBrite NIR?
- A 660/20 filter captures the peak of the LysoBrite NIR emission near 650 nm with minimal spillover.