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Sytox AADvanced

Sytox AADvanced (Ex-Max 544 nm/Em-Max 645 nm)

Sytox AADvanced (7-AAD) from Thermo Fisher Scientific is a cell-impermeant, fluorogenic nucleic acid stain that can be used as a viability dye or to detect apoptosis in flow cytometry. It has an excitation peak at 544 nm and an emission peak at 645 nm.

Excitation & emission spectra

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Key facts

Excitation maximum
544 nm
Emission maximum
645 nm
Optimal laser line
488-532
Optimal filter
630/69
Commercial antibodies
2 from 1 supplier

Sytox AADvanced has an excitation maximum at 544 nm and an emission maximum at 645 nm, a Stokes shift of 101 nm. It is optimally excited by the 488-532 laser line.

Frequently asked questions

What is Sytox AADvanced?
Sytox AADvanced (7-AAD) from Thermo Fisher Scientific is a cell-impermeant, fluorogenic nucleic acid stain that can be used as a viability dye or to detect apoptosis in flow cytometry. It has an excitation peak at 544 nm and an emission peak at 645 nm.
What laser excites Sytox AADvanced?
Sytox AADvanced is optimally excited by the 488-532 laser line. Its excitation maximum is 544 nm.
What filter should I use to detect Sytox AADvanced?
A 630/69 filter captures the peak of the Sytox AADvanced emission near 645 nm with minimal spillover.
How many antibodies are available conjugated to Sytox AADvanced?
FluoroFinder tracks 2 commercially available Sytox AADvanced conjugates, searchable by target, clone, host and application.