Calcein Red AM
Calcein Red AM (Ex-Max 561 nm/Em-Max 571 nm)
Calcein Red, AM, is a fluorogenic red emitting fluorescent molecule (ex 561/ em 571) that penetrates the membranes of live cells. Once inside the live cell, Calcein Red, AM is hydrolyzed by intracellular esterases into the fluorescent Calcein Red which can then be measured via Fluorescence Microscopy, flow cytometer, or fluorescent microplate reader. Reagents that contain an acetoxymethyl ester (AM) or diacetate (DA) side chain, are useful indicators of cell health and vitality, especially when used together with a probe for cell membrane integrity like an impermeant nucleic acid stain or fixable live/dead probe.
Excitation & emission spectra
Key facts
- Excitation maximum
- 561 nm
- Emission maximum
- 571 nm
- Optimal laser line
- 561
- Optimal filter
- 585/42
- Molecular weight
- 1015.79
Calcein Red AM has an excitation maximum at 561 nm and an emission maximum at 571 nm, a Stokes shift of 10 nm. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is Calcein Red AM?
- Calcein Red, AM, is a fluorogenic red emitting fluorescent molecule (ex 561/ em 571) that penetrates the membranes of live cells. Once inside the live cell, Calcein Red, AM is hydrolyzed by intracellular esterases into the fluorescent Calcein Red which can then be measured via Fluorescence Microscopy, flow cytometer, or fluorescent microplate reader. Reagents that contain an acetoxymethyl ester (AM) or diacetate (DA) side chain, are useful indicators of cell health and vitality, especially when used together with a probe for cell membrane integrity like an impermeant nucleic acid stain or fixable live/dead probe.
- What laser excites Calcein Red AM?
- Calcein Red AM is optimally excited by the 561 laser line. Its excitation maximum is 561 nm.
- What filter should I use to detect Calcein Red AM?
- A 585/42 filter captures the peak of the Calcein Red AM emission near 571 nm with minimal spillover.