FVS450
FVS450 (Ex-Max 400 nm/Em-Max 445 nm)
FVS450 (Fixable Viability Stain 450) from BD Biosciences is an amine-reactive viability dye used in flow cytometry when it will be necessary to fix and/or permeabilize the cells after staining. This reagent has an excitation peak at 400 nm and an emission peak at 445 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 400 nm
- Emission maximum
- 445 nm
- Optimal laser line
- 405
- Optimal filter
- 450/50
- Commercial antibodies
- 1 from 1 supplier
FVS450 has an excitation maximum at 400 nm and an emission maximum at 445 nm, a Stokes shift of 45 nm. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is FVS450?
- FVS450 (Fixable Viability Stain 450) from BD Biosciences is an amine-reactive viability dye used in flow cytometry when it will be necessary to fix and/or permeabilize the cells after staining. This reagent has an excitation peak at 400 nm and an emission peak at 445 nm.
- What laser excites FVS450?
- FVS450 is optimally excited by the 405 laser line. Its excitation maximum is 400 nm.
- What filter should I use to detect FVS450?
- A 450/50 filter captures the peak of the FVS450 emission near 445 nm with minimal spillover.
- How many antibodies are available conjugated to FVS450?
- FluoroFinder tracks 1 commercially available FVS450 conjugates, searchable by target, clone, host and application.