Pacific Blue Dye Profile
405

450/50

401

452

About Pacific Blue
Pacific Blue™ (Pac Blue, PacBlue) is a blue-emitting organic fluorophore that can be excited by the 405 nm violet laser and captured with a 450/50 nm bandpass filter. Pacific Blue has an excititaion peak at 401 nm and an emission peak at 452 nm. Pacific Blue remains strongly fluorescent up to neutral pH. This dye has a near identical emission peak to DAPI, so instruments with a DAPI emission filter set will be able to collect Pacific Blue’s emission light. One alternative to Pacific Blue that shares a near identical excitation and emission spectrum is the BD Horizon V450 dye (trademarked by BD Biosciences). Pacific Blue is a trademark of ThermoFisher. This dye comes from the ‘Pacific’ dye product line manufactured by Invitrogen (ThermoFisher) that includes Pacific Blue, Pacific Green, and Pacific Orange. These dyes are often used together because they are all be excited by the same 405 nm violet laser and all three dyes have distinct emission peaks at 455, 500, and 551 nm respectively, so emission peaks do not interfere with each other.
Spectra
Dyes similar to Pacific Blue based on emission
Check to add to spectra viewer4-MUP BFP Calcein Blue AM Calcein Blue Calcein Violet AM CellTrace Violet CellVue Lavender CF350 CF405M CF408 Atlantic Blue DAPI DiFMU pH3.0 DiFMU pH9.0 DY-360XL eFluor 450 eFluor 455UV Fix Viability ExoBrite 410/450 EV Membrane Stain FVS450 FxCycle Violet Ghost Dye Violet 450 Hoechst (33258) iFluor 350 Live/Dead Fix Violet Live-or-Dye 350/448 Live-or-Dye 405/452 Marina Blue Nuclear-ID Blue Nuclear Violet Oxazole Blue Oxazole Blue Homodimer PhenoVue Hoechst 33342 Nuclear Stain POPO-1 ReadiLink 405-454 TagBFP Tag-It Violet V450 ViaFluor 405 Viobility 405/452 Fixable Dye VioBlue violetFluor 450 VivaFix 410/450 Zombie UV
Popular Pacific Blue Antibodies
Company/SKU | Applications | Marker / Reactivity | Host / Isotype / Clone | Size / Price | Details |
---|---|---|---|---|---|
EXBIO PB-638-T025 |
FC,IHC-Fr,ICC,IP | CD20 Human,Non-Human Primate |
Mouse IgG2b 2H7 |
25 Tests $110 |
View |
BioLegend BL-CONJ-PACBLUE |
N/A | Pacific Blue |
N/A N/A N/A |
check prodμcts |
View |
Sony 1103640 |
FC | CD8a Mouse |
Rat IgG2a κ 53-6.7 |
25 μg $98 |
View |
EXBIO PB-684-T025 |
FC,IHC-P,IHC-Fr,WB,ICC,IP | CD45 Human,Non-Human Primate |
Mouse IgG1 κ HI30 |
25 Tests $110 |
View |
EXBIO PB-202-T100 |
CyTOF,FC,IP | CD3 Human |
Mouse IgG2a κ MEM-57 |
100 Tests $220 |
View |
Sony 2366050 |
FC | CCR7 Human,Rhesus,Cynomolgus,Baboon |
Mouse IgG2a κ G043H7 |
100 Tests $318 |
View |
EXBIO PB-160-T100 |
FC | CD45 Human |
Mouse IgG1 κ 2D1 |
100 Tests $220 |
View |
Sony 2101645 |
FC | CD3 Human |
Mouse IgG2a κ HIT3a |
25 Tests $121 |
View |
EXBIO PB-208-T100 |
CyTOF,FC,IHC-P,WB,Functional | CD9 Human |
Mouse IgG1 MEM-61 |
100 Tests $220 |
View |
Sony 2249580 |
FC | PD-1 Human,Chimpanzee,Common Marmoset,Rhesus,Squirrel Monkey,Cynomolgus |
Mouse IgG1 κ EH12.2H7 |
100 μg $318 |
View |
Search Antibodies |
Popular Pacific Blue Secondary Antibodies
Company/SKU | Applications | Marker / Reactivity | Host / Isotype / Clone | Size / Price | Details |
---|---|---|---|---|---|
Beckman Coulter B30656 |
IgM Human |
Mouse IgG1 SA-DA4 |
50 Tests $340 |
View | |
BioLegend 405712 |
FC | IgD Mouse |
Rat IgG2a κ 11-26c.2a |
100 μg $223 |
View |
BioLegend 314514 |
FC | IgM Human,African Green Monkey,Baboon,Cynomolgus,Rhesus |
Mouse IgG1 κ MHM-88 |
100 μg $347 |
View |
BioLegend 405711 |
FC | IgD Mouse |
Rat IgG2a κ 11-26c.2a |
25 μg $106 |
View |
BioLegend 348224 |
FC | IgD Human |
Mouse IgG2a κ IA6-2 |
100 Tests $347 |
View |
Thermo Fisher Scientific P10993 |
FC | IgG Mouse |
Goat IgG Polyclonal |
500 μl $168 |
View |
BioLegend 316616 |
FC | Ig Lambda Light Chain Human,Baboon,Cynomolgus,Rhesus |
Mouse IgG2a κ MHL-38 |
100 μg $300 |
View |
BioLegend 314513 |
FC | IgM Human,African Green Monkey,Baboon,Cynomolgus,Rhesus |
Mouse IgG1 κ MHM-88 |
25 μg $165 |
View |
Thermo Fisher Scientific P10994 |
FC | IgG Rabbit |
Goat IgG Polyclonal |
500 μl $167 |
View |
Sysmex America BF605953 |
FC | Ig Kappa Light Chain Human |
Mouse IgG1 A8B5 |
400 μl $290 |
View |
Search Antibodies |
Pacific Blue Conjugation Kits
Product Type | Company/SKU | Applications | Size / Price | Details |
---|---|---|---|---|
Conjugation Kit |
Thermo Fisher Scientific P30013 |
Labeling | 5 x 100 μg $439 |
View |