Pacific Blue Dye Profile


405

Laser Line

450/50

Common Filter

401

Excitation Max

452

Emission Max


About Pacific Blue


Pacific Blue™ (Pac Blue, PacBlue) is a blue-emitting organic fluorophore that can be excited by the 405 nm violet laser and captured with a 450/50 nm bandpass filter. Pacific Blue has an excititaion peak at 401 nm and an emission peak at 452 nm. Pacific Blue remains strongly fluorescent up to neutral pH. This dye has a near identical emission peak to DAPI, so instruments with a DAPI emission filter set will be able to collect Pacific Blue’s emission light. One alternative to Pacific Blue that shares a near identical excitation and emission spectrum is the BD Horizon V450 dye (trademarked by BD Biosciences). Pacific Blue is a trademark of ThermoFisher. This dye comes from the ‘Pacific’ dye product line manufactured by Invitrogen (ThermoFisher) that includes Pacific Blue, Pacific Green, and Pacific Orange. These dyes are often used together because they are all be excited by the same 405 nm violet laser and all three dyes have distinct emission peaks at 455, 500, and 551 nm respectively, so emission peaks do not interfere with each other.



Spectra


Popular Pacific Blue Antibodies


Company/SKU Applications Marker / Reactivity Host / Isotype / Clone Size / Price Details
EXBIO
PB-638-T025
FC,IHC-Fr,ICC,IP CD20
Human,Non-Human Primate
Mouse
IgG2b
2H7
25 Tests
$110
View
BioLegend
BL-CONJ-PACBLUE
N/A Pacific Blue
N/A
N/A
N/A
check prodμcts
View
Sony
1103640
FC CD8a
Mouse
Rat
IgG2a κ
53-6.7
25 μg
$98
View
EXBIO
PB-684-T025
FC,IHC-P,IHC-Fr,WB,ICC,IP CD45
Human,Non-Human Primate
Mouse
IgG1 κ
HI30
25 Tests
$110
View
EXBIO
PB-202-T100
CyTOF,FC,IP CD3
Human
Mouse
IgG2a κ
MEM-57
100 Tests
$220
View
Sony
2366050
FC CCR7
Human,Rhesus,Cynomolgus,Baboon
Mouse
IgG2a κ
G043H7
100 Tests
$318
View
EXBIO
PB-160-T100
FC CD45
Human
Mouse
IgG1 κ
2D1
100 Tests
$220
View
Sony
2101645
FC CD3
Human
Mouse
IgG2a κ
HIT3a
25 Tests
$121
View
EXBIO
PB-208-T100
CyTOF,FC,IHC-P,WB,Functional CD9
Human
Mouse
IgG1
MEM-61
100 Tests
$220
View
Sony
2249580
FC PD-1
Human,Chimpanzee,Common Marmoset,Rhesus,Squirrel Monkey,Cynomolgus
Mouse
IgG1 κ
EH12.2H7
100 μg
$318
View
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Popular Pacific Blue Secondary Antibodies


Company/SKU Applications Marker / Reactivity Host / Isotype / Clone Size / Price Details
Beckman Coulter
B30656
IgM
Human
Mouse
IgG1
SA-DA4
50 Tests
$340
View
BioLegend
405712
FC IgD
Mouse
Rat
IgG2a κ
11-26c.2a
100 μg
$223
View
BioLegend
314514
FC IgM
Human,African Green Monkey,Baboon,Cynomolgus,Rhesus
Mouse
IgG1 κ
MHM-88
100 μg
$347
View
BioLegend
405711
FC IgD
Mouse
Rat
IgG2a κ
11-26c.2a
25 μg
$106
View
BioLegend
348224
FC IgD
Human
Mouse
IgG2a κ
IA6-2
100 Tests
$347
View
Thermo Fisher Scientific
P10993
FC IgG
Mouse
Goat
IgG
Polyclonal
500 μl
$168
View
BioLegend
316616
FC Ig Lambda Light Chain
Human,Baboon,Cynomolgus,Rhesus
Mouse
IgG2a κ
MHL-38
100 μg
$300
View
BioLegend
314513
FC IgM
Human,African Green Monkey,Baboon,Cynomolgus,Rhesus
Mouse
IgG1 κ
MHM-88
25 μg
$165
View
Thermo Fisher Scientific
P10994
FC IgG
Rabbit
Goat
IgG
Polyclonal
500 μl
$167
View
Sysmex America
BF605953
FC Ig Kappa Light Chain
Human
Mouse
IgG1
A8B5
400 μl
$290
View
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Pacific Blue Conjugation Kits


Product Type Company/SKU Applications Size / Price Details
Conjugation Kit Thermo Fisher Scientific
P30013
Labeling 5 x 100 μg
$439
View

Pacific Blue Conjugation Services


Product Type Company/SKU Applications Details
Conjugation Service BD Biosciences
BD-CONJ-PACBLUE
N/A View
Conjugation Service Columbia Biosciences
COLUMBIA-CONJ-Pacific Blue
Labeling View
Conjugation Service BioLegend
BL-CONJ-PACBLUE
N/A View