About Pacific Blue

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Pacific Blue™ (Pac Blue, PacBlue) is a blue-emitting organic fluorophore that can be excited by the 405 nm violet laser and captured with a 450/50 nm bandpass filter. Pacific Blue has an excititaion peak at 401 nm and an emission peak at 452 nm. Pacific Blue remains strongly fluorescent up to neutral pH. This dye has a near identical emission peak to DAPI, so instruments with a DAPI emission filter set will be able to collect Pacific Blue’s emission light. One alternative to Pacific Blue that shares a near identical excitation and emission spectrum is the BD Horizon V450 dye (trademarked by BD Biosciences). Pacific Blue is a trademark of ThermoFisher. This dye comes from the ‘Pacific’ dye product line manufactured by Invitrogen (ThermoFisher) that includes Pacific Blue, Pacific Green, and Pacific Orange. These dyes are often used together because they are all be excited by the same 405 nm violet laser and all three dyes have distinct emission peaks at 455, 500, and 551 nm respectively, so emission peaks do not interfere with each other.

Dyes similar to Pacific Blue based on emission

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4-MUP BFP Calcein Blue AM Calcein Blue Calcein Violet AM CellTrace Violet CellVue Lavender CF350 CF405M CF408 Atlantic Blue DAPI DiFMU pH3.0 DiFMU pH9.0 DY-360XL eFluor 450 eFluor 455UV Fix Viability ExoBrite 410/450 EV Membrane Stain FVS450 FxCycle Violet Ghost Dye Violet 450 Hoechst (33258) iFluor 350 Live/Dead Fix Violet Live-or-Dye 350/448 Live-or-Dye 405/452 Marina Blue Nuclear-ID Blue Nuclear Violet Oxazole Blue Oxazole Blue Homodimer PhenoVue Hoechst 33342 Nuclear Stain POPO-1 ReadiLink 405-454 TagBFP Tag-It Violet V450 ViaFluor 405 Viobility 405/452 Fixable Dye VioBlue violetFluor 450 VivaFix 410/450 Zombie UV