405

Laser Line

450/50

Common Filter

401

Excitation Max

452

Emission Max

1,437

Antibodies

1

Conjugation Kits

3

Services


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About Pacific Blue


Pacific Blue™ (Pac Blue, PacBlue) is a blue-emitting organic fluorophore that can be excited by the 405 nm violet laser and captured with a 450/50 nm bandpass filter. Pacific Blue has an excititaion peak at 401 nm and an emission peak at 452 nm. Pacific Blue remains strongly fluorescent up to neutral pH. This dye has a near identical emission peak to DAPI, so instruments with a DAPI emission filter set will be able to collect Pacific Blue’s emission light. One alternative to Pacific Blue that shares a near identical excitation and emission spectrum is the BD Horizon V450 dye (trademarked by BD Biosciences). Pacific Blue is a trademark of ThermoFisher. This dye comes from the ‘Pacific’ dye product line manufactured by Invitrogen (ThermoFisher) that includes Pacific Blue, Pacific Green, and Pacific Orange. These dyes are often used together because they are all be excited by the same 405 nm violet laser and all three dyes have distinct emission peaks at 455, 500, and 551 nm respectively, so emission peaks do not interfere with each other.


Popular Pacific Blue Antibodies


Company/SKU Applications Marker / Reactivity Host / Isotype / Clone Size / Price Details
BioLegend
101223
FC CD11b
Mouse,Human,Chimpanzee,Baboon,Cynomolgus,Rhesus,Rabbit
Rat
IgG2b κ
M1/70
25 μg
$94
View
BioLegend
101224
FC CD11b
Mouse,Human,Chimpanzee,Baboon,Cynomolgus,Rhesus,Rabbit
Rat
IgG2b κ
M1/70
100 μg
$215
View
BioLegend
300330
FC CD3
Human
Mouse
IgG2a κ
HIT3a
100 Tests
$248
View
BioLegend
317429
FC CD4
Human,Chimpanzee,Cynomolgus,Rhesus
Mouse
IgG2b κ
OKT4
100 Tests
$264
View
BioLegend
100214
FC CD3
Mouse
Rat
IgG2b κ
17A2
100 μg
$215
View
BioLegend
100428
FC CD4
Mouse
Rat
IgG2b κ
GK1.5
100 μg
$215
View
BioLegend
100725
FC CD8a
Mouse
Rat
IgG2a κ
53-6.7
100 μg
$215
View
BioLegend
103125
FC CD45
Mouse
Rat
IgG2b κ
30-F11
25 μg
$94
View
BioLegend
103126
FC CD45
Mouse
Rat
IgG2b κ
30-F11
100 μg
$215
View
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