VivaFix 353/442
VivaFix 353/442 (Ex-Max 353 nm/Em-Max 442 nm)
VivaFix 353/442 from Bio-Rad is a fixable viability dye useful in flow cytometry in applications where the cells will be fixed and permeabilized. It has an excitation peak at 353 nm and an emission peak at 442 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 353 nm
- Emission maximum
- 442 nm
- Optimal laser line
- 355
- Optimal filter
- 450/50
VivaFix 353/442 has an excitation maximum at 353 nm and an emission maximum at 442 nm, a Stokes shift of 89 nm. It is optimally excited by the 355 laser line.
Frequently asked questions
- What is VivaFix 353/442?
- VivaFix 353/442 from Bio-Rad is a fixable viability dye useful in flow cytometry in applications where the cells will be fixed and permeabilized. It has an excitation peak at 353 nm and an emission peak at 442 nm.
- What laser excites VivaFix 353/442?
- VivaFix 353/442 is optimally excited by the 355 laser line. Its excitation maximum is 353 nm.
- What filter should I use to detect VivaFix 353/442?
- A 450/50 filter captures the peak of the VivaFix 353/442 emission near 442 nm with minimal spillover.