CF405M
CF®405M (Ex-Max 408 nm/Em-Max 452 nm)
CF®405M from Biotium is a fluorophore with an excitation peak at 408 nm and an emission peak at 452 nm. It is spectrally similar to Pacific Blue, V450 and DY-415. It can be use in fluorescence microscopy and flow cytometry. Specifically, it has been validated for use in STED, Structured Illumination (SIM) and 2-Photon microscopy. In flow cytometry, superior alternatives are Brilliant™ Violet 480, Brilliant™ Violet 510 and StarBright Violet 515.
Excitation & emission spectra
Key facts
- Excitation maximum
- 408 nm
- Emission maximum
- 452 nm
- Optimal laser line
- 405
- Optimal filter
- 450/50
- Relative brightness
- 2 of 5
- Extinction coefficient
- 41000
- Molecular weight
- 503
- Commercial conjugates
- 71 from 5 suppliers
CF405M has an excitation maximum at 408 nm and an emission maximum at 452 nm, a Stokes shift of 44 nm. Its extinction coefficient is 41000 M⁻¹cm⁻¹. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is CF405M?
- CF®405M from Biotium is a fluorophore with an excitation peak at 408 nm and an emission peak at 452 nm. It is spectrally similar to Pacific Blue, V450 and DY-415. It can be use in fluorescence microscopy and flow cytometry. Specifically, it has been validated for use in STED, Structured Illumination (SIM) and 2-Photon microscopy. In flow cytometry, superior alternatives are Brilliant™ Violet 480, Brilliant™ Violet 510 and StarBright Violet 515.
- What laser excites CF405M?
- CF405M is optimally excited by the 405 laser line. Its excitation maximum is 408 nm.
- What filter should I use to detect CF405M?
- A 450/50 filter captures the peak of the CF405M emission near 452 nm with minimal spillover.
- How bright is CF405M?
- CF405M has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to CF405M?
- FluoroFinder tracks 71 commercially available CF405M conjugates, searchable by target, clone, host and application.