AMCA
AMCA (Coumarin)
AMCA (AMC, Coumarin) is a fluorophore with an excitation peak at 344 nm and an emission peak at 440 nm. Coumarin is the core blue-emitting fluorophore that has derived other, more stable alternatives like Alexa Fluor™ 350 and DY350.
Excitation & emission spectra
Key facts
- Excitation maximum
- 344 nm
- Emission maximum
- 439 nm
- Optimal laser line
- 355
- Optimal filter
- 450/50
- Relative brightness
- 1 of 5
- Commercial antibodies
- 21 from 10 suppliers
AMCA has an excitation maximum at 344 nm and an emission maximum at 439 nm, a Stokes shift of 95 nm. It is optimally excited by the 355 laser line.
Frequently asked questions
- What is AMCA?
- AMCA (AMC, Coumarin) is a fluorophore with an excitation peak at 344 nm and an emission peak at 440 nm. Coumarin is the core blue-emitting fluorophore that has derived other, more stable alternatives like Alexa Fluor™ 350 and DY350.
- What laser excites AMCA?
- AMCA is optimally excited by the 355 laser line. Its excitation maximum is 344 nm.
- What filter should I use to detect AMCA?
- A 450/50 filter captures the peak of the AMCA emission near 439 nm with minimal spillover.
- How bright is AMCA?
- AMCA has a relative brightness of 1 out of 5.
- How many antibodies are available conjugated to AMCA?
- FluoroFinder tracks 21 commercially available AMCA conjugates, searchable by target, clone, host and application.