Loading the Dye Directory…

VivaFix 410/450

VivaFix 410/450 (Ex-Max 410 nm/Em-Max 450 nm)

VivaFix 410/450 from Bio-Rad is a fixable viability dye useful in flow cytometry in applications where the cells will be fixed and permeabilized. It has an excitation peak at 410 nm and an emission peak at 450 nm.

Excitation & emission spectra

400450500550600650700750800

Key facts

Excitation maximum
410 nm
Emission maximum
450 nm
Optimal laser line
405
Optimal filter
450/50
Commercial antibodies
1 from 1 supplier

VivaFix 410/450 has an excitation maximum at 410 nm and an emission maximum at 450 nm, a Stokes shift of 40 nm. It is optimally excited by the 405 laser line.

Frequently asked questions

What is VivaFix 410/450?
VivaFix 410/450 from Bio-Rad is a fixable viability dye useful in flow cytometry in applications where the cells will be fixed and permeabilized. It has an excitation peak at 410 nm and an emission peak at 450 nm.
What laser excites VivaFix 410/450?
VivaFix 410/450 is optimally excited by the 405 laser line. Its excitation maximum is 410 nm.
What filter should I use to detect VivaFix 410/450?
A 450/50 filter captures the peak of the VivaFix 410/450 emission near 450 nm with minimal spillover.
How many antibodies are available conjugated to VivaFix 410/450?
FluoroFinder tracks 1 commercially available VivaFix 410/450 conjugates, searchable by target, clone, host and application.