About Live/Dead Fix Violet

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The Live/Dead Fixable Viability probes from Thermo Fisher are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live/Dead Fixable Violet has an excitation peak at 402 nm and an emission peak at 452 nm.

Alternative Dyes to Live/Dead Fix Violet

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Ghost Dye Violet 450 FVS450 VivaFix 410/450

Dyes similar to Live/Dead Fix Violet based on emission

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4-MUP Atlantic Blue BFP Calcein Blue AM Calcein Blue Calcein Violet AM CellTrace Violet CellVue Lavender CF350 CF405M CF408 DAPI DiFMU pH3.0 DiFMU pH9.0 DY-360XL eFluor 450 eFluor 455UV Fix Viability ExoBrite 410/450 EV Membrane Stain FVS450 FxCycle Violet Ghost Dye Violet 450 Hoechst (33258) iFluor 350 Live-or-Dye 350/448 Live-or-Dye 405/452 Marina Blue Nuclear-ID Blue Nuclear Violet Oxazole Blue Oxazole Blue Homodimer Pacific Blue PhenoVue DAPI Nuclear Stain PhenoVue Hoechst 33342 Nuclear Stain POPO-1 ReadiLink 405-454 TagBFP Tag-It Violet V450 ViaFluor 405 Viobility 405/452 Fixable Dye Vio Blue violetFluor 450 VivaFix 410/450 Zombie UV PhenoVue Fluor 405 CytoLiner 410/450 RayBright Violet 450 Alexa Fluor Plus 405 ABflo 450 CF410 ExoBrite 400/460 True EV Membrane Stain 7-hydroxy-4-methylcoumarin