About Calcein Blue AM

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Calcein Blue, AM, is a fluorogenic blue fluorescent molecule (ex 359/ em 446) that penetrates the membranes of live cells. Once inside the live cell, Calcein Blue, AM is hydrolyzed by intracellular esterases into the fluorescent Calcein Blue which can then be measured via Fluorescence Microscopy, flow cytometer, or fluorescent microplate reader. Reagents that contain an acetoxymethyl ester (AM) or diacetate (DA) side chain, are useful indicators of cell health and vitality, especially when used together with a probe for cell membrane integrity like an impermeant nucleic acid stain or fixable live/dead probe.

Dyes similar to Calcein Blue AM based on emission

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4-MUP 7-Amino-4-methylcoumarin (AMC) Alexa Fluor 350 AMCA BFP Calcein Blue Calcein Violet AM CellBrite Blue CellBrite Blue CellTrace Violet CF350 CF405M CF408 CytoTrack Blue DiFMU pH3.0 DiFMU pH9.0 eBFP eFluor 450 Cell Proliferation eFluor 450 eFluor 450 Fix Viability eFluor 455UV Fix Viability ExoBrite 410/450 EV Membrane Stain FVS450 Ghost Dye Violet 450 Hoechst (33258) Hoechst (34580) iFluor 350 Live/Dead Fix Blue Live/Dead Fix Violet Live-or-Dye 350/448 Live-or-Dye 405/452 LysoSensor Yellow/Blue pH9.0 MitoView 405 Pacific Blue PhenoVue Hoechst 33342 Nuclear Stain ReadiLink 405-454 StarBright UltraViolet 445 TagBFP Tag-It Violet V450 ViaFluor 405 Viobility 405/452 Fixable Dye Vio Blue violetFluor 450 VivaFix 353/442 VivaFix 410/450 Vybrant DyeCycle Violet Zombie UV RayBright Violet 450 ABflo 450 7-hydroxy-4-methylcoumarin CF410 Aco-430 XFD350 PhenoVue Fluor 405 AbBy Fluor 350 Alexa Fluor Plus 405 CytoLiner 410/450