4-MUP
4-MUP (4-Methylumbelliferyl phosphate) is a substrate for alkaline phosphatase which forms the fluorescent methylumbelliferone and can be used in ELISA and SEAP assays.
Excitation & emission spectra
Key facts
- Excitation maximum
- 359 nm
- Emission maximum
- 445 nm
- Optimal laser line
- 355
- Optimal filter
- 450/50
- Molecular weight
- 256.15
4-MUP has an excitation maximum at 359 nm and an emission maximum at 445 nm, a Stokes shift of 86 nm. It is optimally excited by the 355 laser line.
Frequently asked questions
- What is 4-MUP?
- 4-MUP (4-Methylumbelliferyl phosphate) is a substrate for alkaline phosphatase which forms the fluorescent methylumbelliferone and can be used in ELISA and SEAP assays.
- What laser excites 4-MUP?
- 4-MUP is optimally excited by the 355 laser line. Its excitation maximum is 359 nm.
- What filter should I use to detect 4-MUP?
- A 450/50 filter captures the peak of the 4-MUP emission near 445 nm with minimal spillover.