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SuperBright 436
SuperBright 436 (Ex-Max 414 nm/Em-Max 436 nm)
SuperBright 436 (SB436) is a fluorescent polymer with an excitation peak at 414 nm and an emission peak at 436 nm. SB436 is spectrally similar to StarBright Violet 440, eFluor™ 450 and Pacific Blue but can be unmixed with Brilliant™ Violet 421 on a spectral analyzer. This dye is a member of the SuperBright dye family, sold by ThermoFisher Scientific under license from BD Bioscience.
Excitation & emission spectra
Key facts
- Excitation maximum
- 414 nm
- Emission maximum
- 436 nm
- Optimal laser line
- 405
- Optimal filter
- 450/50
- Relative brightness
- 4 of 5
- Commercial antibodies
- 289 from 2 suppliers
SuperBright 436 has an excitation maximum at 414 nm and an emission maximum at 436 nm, a Stokes shift of 22 nm. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is SuperBright 436?
- SuperBright 436 (SB436) is a fluorescent polymer with an excitation peak at 414 nm and an emission peak at 436 nm. SB436 is spectrally similar to StarBright Violet 440, eFluor™ 450 and Pacific Blue but can be unmixed with Brilliant™ Violet 421 on a spectral analyzer. This dye is a member of the SuperBright dye family, sold by ThermoFisher Scientific under license from BD Bioscience.
- What laser excites SuperBright 436?
- SuperBright 436 is optimally excited by the 405 laser line. Its excitation maximum is 414 nm.
- What filter should I use to detect SuperBright 436?
- A 450/50 filter captures the peak of the SuperBright 436 emission near 436 nm with minimal spillover.
- How bright is SuperBright 436?
- SuperBright 436 has a relative brightness of 4 out of 5.
- How many antibodies are available conjugated to SuperBright 436?
- FluoroFinder tracks 289 commercially available SuperBright 436 conjugates, searchable by target, clone, host and application.