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Vybrant DyeCycle Violet

Vybrant DyeCycle Violet (Ex-Max 369 nm/Em-Max 437 nm)

Vybrant DyeCycle Violet from Thermo Fisher Scientific is a fluorogenic, cell-permeant nucleic acid stain that can be used to assess cell cycle in live or fixed cells. Vybrant DyeCycle probes are an alternative to the CytoPhase probes from Biolegend. It has an excitation peak at 369 nm and an emission peak at 437 nm.

Excitation & emission spectra

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Key facts

Excitation maximum
369 nm
Emission maximum
437 nm
Optimal laser line
355
Optimal filter
450/50

Vybrant DyeCycle Violet has an excitation maximum at 369 nm and an emission maximum at 437 nm, a Stokes shift of 68 nm. It is optimally excited by the 355 laser line.

Frequently asked questions

What is Vybrant DyeCycle Violet?
Vybrant DyeCycle Violet from Thermo Fisher Scientific is a fluorogenic, cell-permeant nucleic acid stain that can be used to assess cell cycle in live or fixed cells. Vybrant DyeCycle probes are an alternative to the CytoPhase probes from Biolegend. It has an excitation peak at 369 nm and an emission peak at 437 nm.
What laser excites Vybrant DyeCycle Violet?
Vybrant DyeCycle Violet is optimally excited by the 355 laser line. Its excitation maximum is 369 nm.
What filter should I use to detect Vybrant DyeCycle Violet?
A 450/50 filter captures the peak of the Vybrant DyeCycle Violet emission near 437 nm with minimal spillover.