eFluor 450 Cell Proliferation
eFluor™ 450 Cell Proliferation (Ex-Max 402 nm/Em-Max 442 nm)
eFluor™ 450 Cell Proliferation is passively cell permeant. Inside the cell it is retained by intracellular esterase activity and conjugates to intracellular proteins to aid retention. It can be used to monitor cellular division of multiple daughter generations. It has an excitation peak at 402 nm and an emission peak at 442 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 402 nm
- Emission maximum
- 442 nm
- Optimal laser line
- 405
- Optimal filter
- 450/50
eFluor 450 Cell Proliferation has an excitation maximum at 402 nm and an emission maximum at 442 nm, a Stokes shift of 40 nm. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is eFluor 450 Cell Proliferation?
- eFluor™ 450 Cell Proliferation is passively cell permeant. Inside the cell it is retained by intracellular esterase activity and conjugates to intracellular proteins to aid retention. It can be used to monitor cellular division of multiple daughter generations. It has an excitation peak at 402 nm and an emission peak at 442 nm.
- What laser excites eFluor 450 Cell Proliferation?
- eFluor 450 Cell Proliferation is optimally excited by the 405 laser line. Its excitation maximum is 402 nm.
- What filter should I use to detect eFluor 450 Cell Proliferation?
- A 450/50 filter captures the peak of the eFluor 450 Cell Proliferation emission near 442 nm with minimal spillover.