CellBrite Blue Dye Profile
About CellBrite Blue
Biotium’s CellBrite Fix dyes are versatile amine-reactive dyes that are primarily used for live cell outer plasma membrane staining but can withstand fixation and detergent permeabilization. The unique benefit here is that since CellBrite Fix dyes can withstand permeabilization, extracellular staining can be combined with intracellular staining techniques. This dye line shares similarities with Biotium’s MemBrite Fix and MemBrite Fix-ST line, but there are a few key differences. CellBrite Fix stains are easy to use and require a single staining step while MemBrite Fix dyes require a two-step staining protocol. While ease of use is important, MemBrite Fix and Membrite Fix-ST dyes have more colors to choose from and they do not associate with lipids in the membrane which leads to lower cytoplasmic background after permeabilization.
Dyes similar to CellBrite Blue based on emissionCheck to add to spectra viewer
4-MUP 7-Amino-4-methylcoumarin (AMC) Alexa Fluor 350 AMCA BFP Calcein Blue AM Calcein Blue Calcein Violet AM CellBrite Blue CellTrace Violet CF350 CF408 Atlantic Blue CytoTrack Blue DiFMU pH3.0 DiFMU pH9.0 DyLight 350 eBFP eFluor 450 Cell Proliferation eFluor 450 eFluor 450 Fix Viability ExoBrite 410/450 EV Membrane Stain FVS440UV FVS450 Ghost Dye UV 450 Ghost Dye Violet 450 Hoechst (34580) iFluor 350 Live/Dead Fix Blue Live-or-Dye 350/448 MitoView 405 StarBright UltraViolet 445 StarBright Violet 440 SuperBright 436 V450 violetFluor 450 VivaFix 353/442 VivaFix 410/450 Vybrant DyeCycle Violet