CytoTrack Blue
CytoTrack Blue (Ex-Max 409 nm/Em-Max 440 nm)
CytoTrack Blue (403/454) from Bio-Rad passively crosses the cell membrane and is hydrolyzed causing it to become fluorescent and retained intracellularly. It then covalently crosslinks with primary amine-containing proteins inside the cell. With each daughter generation, as the cell divides, the intensity of the dye will become half of it's parent, indicating a symmetrical division of the cell and cytoplasm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 409 nm
- Emission maximum
- 440 nm
- Optimal laser line
- 405
- Optimal filter
- 450/50
CytoTrack Blue has an excitation maximum at 409 nm and an emission maximum at 440 nm, a Stokes shift of 31 nm. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is CytoTrack Blue?
- CytoTrack Blue (403/454) from Bio-Rad passively crosses the cell membrane and is hydrolyzed causing it to become fluorescent and retained intracellularly. It then covalently crosslinks with primary amine-containing proteins inside the cell. With each daughter generation, as the cell divides, the intensity of the dye will become half of it's parent, indicating a symmetrical division of the cell and cytoplasm.
- What laser excites CytoTrack Blue?
- CytoTrack Blue is optimally excited by the 405 laser line. Its excitation maximum is 409 nm.
- What filter should I use to detect CytoTrack Blue?
- A 450/50 filter captures the peak of the CytoTrack Blue emission near 440 nm with minimal spillover.