V450 Dye Profile
405

450/40

404

448

Dyes similar to V450 based on emission
4-MUP 7-Amino-4-methylcoumarin (AMC) Alexa Fluor 350 AMCA BFP Calcein Blue AM Calcein Blue Calcein Violet AM CellBrite Blue CellBrite Blue CellTrace Violet CellVue Lavender CF350 CF405M CF408 Atlantic Blue CytoTrack Blue DAPI DiFMU pH3.0 DiFMU pH9.0 DY-360XL eBFP eFluor 450 Cell Proliferation eFluor 450 eFluor 450 Fix Viability eFluor 455UV Fix Viability ExoBrite 410/450 EV Membrane Stain FVS450 Ghost Dye Violet 450 Hoechst (33258) Hoechst (34580) iFluor 350 Live/Dead Fix Blue Live/Dead Fix Violet Live-or-Dye 350/448 Live-or-Dye 405/452 MitoView 405 Oxazole Blue Oxazole Blue Homodimer Pacific Blue PhenoVue Hoechst 33342 Nuclear Stain POPO-1 ReadiLink 405-454 StarBright UltraViolet 445 TagBFP Tag-It Violet ViaFluor 405 Viobility 405/452 Fixable Dye VioBlue violetFluor 450 VivaFix 353/442 VivaFix 410/450 Zombie UVAbout V450
V450 (BD Horizon V450) is a blue-emitting coumarin fluorophore that can be excited by the 405 nm violet laser and captured with a 450/40 nm bandpass filter. V450 has an excitation peak at 406 nm and an emission peak at 450 nm, and is spectrally similar to eFluor™ 450 (ThermoFisher Scientific), VioBlue® (Miltenyi Biotec), Alexa Fluor 405 (ThermoFisher Scientific), Cascade Blue™ (ThermoFisher Scientific), Pacific Blue (ThermoFisher Scientific), SuperBright 436 (ThermoFisher Scientific), ATTO 425 (Atto-Tec) V450 is most commonly used in flow cytometry. Coumarin is a naturally occurring small molecular weight compound in plants that when modified forms the basis of various fluorescent probes. These fluorescent probes share some similar properties, such as being UV excitable and blue light emitting, while probes can exhibit differences such as in cell permeability. V450 is a product of BD Biosciences.
Popular V450 Antibodies
Company/SKU | Applications | Marker / Reactivity | Host / Isotype / Clone | Size / Price | Details |
---|---|---|---|---|---|
MBL International FP10254010 |
FC | CD3 Human |
Mouse IgG2a κ OKT3 |
100 Tests $246 |
View |
MBL International FP10264010 |
FC | CD3 Human |
Mouse IgG1 κ UCHT1 |
100 Tests $246 |
View |
MBL International FP10324010 |
FC | CD4 Human |
Mouse IgG2b κ OKT4 |
100 Tests $246 |
View |
MBL International FP10644010 |
FC | CD19 Human |
Mouse IgG1 κ HIB19 |
100 Tests $246 |
View |
MBL International FP10254002 |
FC | CD3 Human |
Mouse IgG2a κ OKT3 |
25 Tests $114 |
View |
MBL International FP10264002 |
FC | CD3 Human |
Mouse IgG1 κ UCHT1 |
25 Tests $102 |
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MBL International FP20274010 |
FC | CD45 Mouse |
Rat IgG2b κ 30-F11 |
100 μg $108 |
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MBL International FP10644002 |
FC | CD19 Human |
Mouse IgG1 κ HIB19 |
25 Tests $114 |
View |
MBL International FP20864010 |
FC | MHC II Mouse |
Rat IgG2b κ M5/114.15.2 |
100 μg $210 |
View |
MBL International FP20784002 |
FC | Ly-6G Mouse |
Rat IgG2b κ RB6-8C5 |
25 μg $96 |
View |
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Popular V450 Secondary Antibodies
Company/SKU | Applications | Marker / Reactivity | Host / Isotype / Clone | Size / Price | Details |
---|---|---|---|---|---|
BD Biosciences 561309 |
FC | IgD Human |
Mouse IgG2a κ IA6-2 |
50 Tests $220 |
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BD Biosciences 561299 |
FC | IgG Human |
Mouse IgG1 κ G18-145 |
50 Tests $245 |
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BD Biosciences 560869 |
FC | IgD Mouse |
Rat IgG2a κ 11-26c.2a |
50 μg $150 |
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BD Biosciences 561286 |
FC | IgM Human |
Mouse IgG1 κ G20-127 |
50 Tests $225 |
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BD Biosciences 562107 |
FC | IgG1 Mouse |
Rat IgG1 κ A85-1 |
50 μg $130 |
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BD Biosciences 560575 |
FC | IgM Mouse |
Rat IgG2a κ R6-60.2 |
50 μg $155 |
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BD Biosciences 658287 |
FC | Ig Kappa Light Chain Human |
Mouse IgG1 κ TB28-2 |
100 Tests $550 |
View |
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V450 Conjugation Services
Product Type | Company/SKU | Applications | Details |
---|---|---|---|
Conjugation Service |
BD Biosciences BD-CONJ-HZV450 |
N/A | View |