V450
V450 (Ex-Max 404 nm/Em-Max 448 nm)
V450 (Horizon V450) from BD Biosciences has an excitation peak at 406 nm and an emission peak at 450 nm. It is spectrally similar to eFluor™ 450 (ThermoFisher Scientific), Vio®Blue® (Miltenyi Biotec), Alexa Fluor™ 405 (ThermoFisher Scientific), Cascade Blue® (ThermoFisher Scientific), Pacific Blue (ThermoFisher Scientific), and ATTO 425 (ATTO-Tec)
Excitation & emission spectra
Key facts
- Excitation maximum
- 404 nm
- Emission maximum
- 448 nm
- Optimal laser line
- 405
- Optimal filter
- 450/40
- Relative brightness
- 2 of 5
- Extinction coefficient
- 38500
- Commercial antibodies
- 336 from 4 suppliers
V450 has an excitation maximum at 404 nm and an emission maximum at 448 nm, a Stokes shift of 44 nm. Its extinction coefficient is 38500 M⁻¹cm⁻¹. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is V450?
- V450 (Horizon V450) from BD Biosciences has an excitation peak at 406 nm and an emission peak at 450 nm. It is spectrally similar to eFluor™ 450 (ThermoFisher Scientific), Vio®Blue® (Miltenyi Biotec), Alexa Fluor™ 405 (ThermoFisher Scientific), Cascade Blue® (ThermoFisher Scientific), Pacific Blue (ThermoFisher Scientific), and ATTO 425 (ATTO-Tec)
- What laser excites V450?
- V450 is optimally excited by the 405 laser line. Its excitation maximum is 404 nm.
- What filter should I use to detect V450?
- A 450/40 filter captures the peak of the V450 emission near 448 nm with minimal spillover.
- How bright is V450?
- V450 has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to V450?
- FluoroFinder tracks 336 commercially available V450 conjugates, searchable by target, clone, host and application.