About Calcein Violet AM

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Calcein Violet is a blue fluorescent molecule that penetrates the membranes of dead cells and is used to evaluate cell membrane integrity as well as esterase activity. Closely related is Calcein Violet AM, which due to the acetoxymethyl (AM) modification, can passively diffuse across the membranes of live cells. While inside the live cell, Calcein Violet AM is hydrolyzed by esterase into the fluorescent Calcein Violet which can then be measured via Fluorescence Microscopy, flow cytometer, or fluorescent microplate reader. It has an excitation peak at 399 nm and an emission peak at 449 nm.

Dyes similar to Calcein Violet AM based on emission

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4-MUP 7-Amino-4-methylcoumarin (AMC) Alexa Fluor 350 Atlantic Blue BFP Calcein Blue AM Calcein Blue CellBrite Blue CellBrite Blue CellTrace Violet CF350 CF405M CF408 CytoTrack Blue DAPI DiFMU pH3.0 DiFMU pH9.0 DY-360XL eBFP eFluor 450 Cell Proliferation eFluor 450 eFluor 450 Fix Viability eFluor 455UV Fix Viability ExoBrite 410/450 EV Membrane Stain FVS450 Ghost Dye Violet 450 Hoechst (33258) Hoechst (34580) iFluor 350 Live/Dead Fix Blue Live/Dead Fix Violet Live-or-Dye 350/448 Live-or-Dye 405/452 LysoSensor Yellow/Blue pH9.0 MitoView 405 Oxazole Blue Oxazole Blue Homodimer Pacific Blue PhenoVue DAPI Nuclear Stain PhenoVue Hoechst 33342 Nuclear Stain POPO-1 ReadiLink 405-454 TagBFP Tag-It Violet V450 ViaFluor 405 Viobility 405/452 Fixable Dye Vio Blue violetFluor 450 VivaFix 353/442 VivaFix 410/450 Zombie UV XFD350 CytoLiner 410/450 AbBy Fluor 350 PhenoVue Fluor 405 Alexa Fluor Plus 405 CF410 ABflo 450 7-hydroxy-4-methylcoumarin RayBright Violet 450 Aco-430