Calcein Violet AM
Calcein Violet AM (Ex-Max 399 nm/Em-Max 449 nm)
Calcein Violet is a blue fluorescent molecule that penetrates the membranes of dead cells and is used to evaluate cell membrane integrity as well as esterase activity. Closely related is Calcein Violet AM, which due to the acetoxymethyl (AM) modification, can passively diffuse across the membranes of live cells. While inside the live cell, Calcein Violet AM is hydrolyzed by esterase into the fluorescent Calcein Violet which can then be measured via Fluorescence Microscopy, flow cytometer, or fluorescent microplate reader. It has an excitation peak at 399 nm and an emission peak at 449 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 399 nm
- Emission maximum
- 449 nm
- Optimal laser line
- 405
- Optimal filter
- 450/50
Calcein Violet AM has an excitation maximum at 399 nm and an emission maximum at 449 nm, a Stokes shift of 50 nm. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is Calcein Violet AM?
- Calcein Violet is a blue fluorescent molecule that penetrates the membranes of dead cells and is used to evaluate cell membrane integrity as well as esterase activity. Closely related is Calcein Violet AM, which due to the acetoxymethyl (AM) modification, can passively diffuse across the membranes of live cells. While inside the live cell, Calcein Violet AM is hydrolyzed by esterase into the fluorescent Calcein Violet which can then be measured via Fluorescence Microscopy, flow cytometer, or fluorescent microplate reader. It has an excitation peak at 399 nm and an emission peak at 449 nm.
- What laser excites Calcein Violet AM?
- Calcein Violet AM is optimally excited by the 405 laser line. Its excitation maximum is 399 nm.
- What filter should I use to detect Calcein Violet AM?
- A 450/50 filter captures the peak of the Calcein Violet AM emission near 449 nm with minimal spillover.