eFluor 450 Fix Viability
eFluor™ 450 Fix Viability (Ex-Max 402 nm/Em-Max 442 nm)
eFluor™ 450 Fixable Viability from Thermo Fisher Scientific is an amine-reactive dye that can be used to discriminate between live and dead cells in flow cytometry. It is compatible with further cryopreservation, fixation or permeabilization. It has an excitaiton peak at 402 nm and an emission peak at 442 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 402 nm
- Emission maximum
- 442 nm
- Optimal laser line
- 405
- Optimal filter
- 450/50
eFluor 450 Fix Viability has an excitation maximum at 402 nm and an emission maximum at 442 nm, a Stokes shift of 40 nm. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is eFluor 450 Fix Viability?
- eFluor™ 450 Fixable Viability from Thermo Fisher Scientific is an amine-reactive dye that can be used to discriminate between live and dead cells in flow cytometry. It is compatible with further cryopreservation, fixation or permeabilization. It has an excitaiton peak at 402 nm and an emission peak at 442 nm.
- What laser excites eFluor 450 Fix Viability?
- eFluor 450 Fix Viability is optimally excited by the 405 laser line. Its excitation maximum is 402 nm.
- What filter should I use to detect eFluor 450 Fix Viability?
- A 450/50 filter captures the peak of the eFluor 450 Fix Viability emission near 442 nm with minimal spillover.