Live-or-Dye 350/448
Live-or-Dye™ 350/448 (Ex-Max 355 nm/Em-Max 450 nm)
Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 350/448 has an excitation peak at 355 nm and an emission peak at 448 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 355 nm
- Emission maximum
- 450 nm
- Optimal laser line
- 355
- Optimal filter
- 450/50
- Commercial antibodies
- 2 from 1 supplier
Live-or-Dye 350/448 has an excitation maximum at 355 nm and an emission maximum at 450 nm, a Stokes shift of 95 nm. It is optimally excited by the 355 laser line.
Frequently asked questions
- What is Live-or-Dye 350/448?
- Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 350/448 has an excitation peak at 355 nm and an emission peak at 448 nm.
- What laser excites Live-or-Dye 350/448?
- Live-or-Dye 350/448 is optimally excited by the 355 laser line. Its excitation maximum is 355 nm.
- What filter should I use to detect Live-or-Dye 350/448?
- A 450/50 filter captures the peak of the Live-or-Dye 350/448 emission near 450 nm with minimal spillover.
- How many antibodies are available conjugated to Live-or-Dye 350/448?
- FluoroFinder tracks 2 commercially available Live-or-Dye 350/448 conjugates, searchable by target, clone, host and application.