355

Laser Line

450/50

Common Filter

359

Excitation Max

445

Emission Max


Spectra Viewer

About Calcein Blue


Calcein Blue is a blue fluorescent molecule that penetrates the membranes of dead cells and is used to evaluate cell membrane integrity as well as esterase activity. Closely related is Calcein Blue AM, which due to the acetoxymethyl (AM) modification, can passively diffuse across the membranes of live cells. While inside the live cell, Calcein Blue AM is hydrolyzed by esterase into the fluorescent Calcein Blue which can then be measured via fluorescent microscopy, flow cytometer, or fluorescent microplate reader.