Calcein Blue Dye Profile
355
Laser Line
450/50
Common Filter
359
Excitation Max
445
Emission Max
Dyes similar to Calcein Blue based on emission
4-MUP 7-Amino-4-methylcoumarin (AMC) Alexa Fluor 350 AMCA BFP Calcein Blue AM Calcein Violet AM CellBrite Blue CellBrite Blue CellTrace Violet CF350 CF405M CF408 Atlantic Blue CytoTrack Blue DiFMU pH3.0 DiFMU pH9.0 eBFP eFluor 450 Cell Proliferation eFluor 450 eFluor 450 Fix Viability ExoBrite 410/450 EV Membrane Stain FVS450 Ghost Dye Violet 450 Hoechst (33258) Hoechst (34580) iFluor 350 Live/Dead Fix Blue Live/Dead Fix Violet Live-or-Dye 350/448 Live-or-Dye 405/452 MitoView 405 Pacific Blue PhenoVue Hoechst 33342 Nuclear Stain ReadiLink 405-454 StarBright UltraViolet 445 StarBright Violet 440 SuperBright 436 V450 ViaFluor 405 Viobility 405/452 Fixable Dye VioBlue violetFluor 450 VivaFix 353/442 VivaFix 410/450 Vybrant DyeCycle Violet Zombie UVAbout Calcein Blue
Calcein Blue is a blue fluorescent molecule that penetrates the membranes of dead cells and is used to evaluate cell membrane integrity as well as esterase activity. Closely related is Calcein Blue AM, which due to the acetoxymethyl (AM) modification, can passively diffuse across the membranes of live cells. While inside the live cell, Calcein Blue AM is hydrolyzed by esterase into the fluorescent Calcein Blue which can then be measured via fluorescent microscopy, flow cytometer, or fluorescent microplate reader.
