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Zombie UV

Zombie UV™ (Ex-Max 360 nm/Em-Max 452 nm)

Zombie UV™ from Biolegend is an amine-reactive viability dye useful in flow cytometry when fixation and permeabilization of the cells is required prior to analysis. It has an excitation peak at 360 nm and an emission peak at 452 nm.

Excitation & emission spectra

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Key facts

Excitation maximum
360 nm
Emission maximum
452 nm
Optimal laser line
355
Optimal filter
450/50

Zombie UV has an excitation maximum at 360 nm and an emission maximum at 452 nm, a Stokes shift of 92 nm. It is optimally excited by the 355 laser line.

Frequently asked questions

What is Zombie UV?
Zombie UV™ from Biolegend is an amine-reactive viability dye useful in flow cytometry when fixation and permeabilization of the cells is required prior to analysis. It has an excitation peak at 360 nm and an emission peak at 452 nm.
What laser excites Zombie UV?
Zombie UV is optimally excited by the 355 laser line. Its excitation maximum is 360 nm.
What filter should I use to detect Zombie UV?
A 450/50 filter captures the peak of the Zombie UV emission near 452 nm with minimal spillover.