Vio Blue
Vio®Blue (Ex-Max 400 nm/Em-Max 452 nm)
Vio®Blue® has an excitation peak at 400 nm and an emission peak at 455 nm, and is spectrally similar to eFluor™ 450 (ThermoFisher Scientific), V450 (BD Biosciences), Alexa Fluor™ 405 (ThermoFisher), Pacific Blue (ThermoFisher Scientific) and ATTO 425 (AttoTec).
Excitation & emission spectra
Key facts
- Excitation maximum
- 400 nm
- Emission maximum
- 452 nm
- Optimal laser line
- 405
- Optimal filter
- 450/50
- Relative brightness
- 2 of 5
- Commercial antibodies
- 678 from 2 suppliers
Vio Blue has an excitation maximum at 400 nm and an emission maximum at 452 nm, a Stokes shift of 52 nm. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is Vio Blue?
- Vio®Blue® has an excitation peak at 400 nm and an emission peak at 455 nm, and is spectrally similar to eFluor™ 450 (ThermoFisher Scientific), V450 (BD Biosciences), Alexa Fluor™ 405 (ThermoFisher), Pacific Blue (ThermoFisher Scientific) and ATTO 425 (AttoTec).
- What laser excites Vio Blue?
- Vio Blue is optimally excited by the 405 laser line. Its excitation maximum is 400 nm.
- What filter should I use to detect Vio Blue?
- A 450/50 filter captures the peak of the Vio Blue emission near 452 nm with minimal spillover.
- How bright is Vio Blue?
- Vio Blue has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to Vio Blue?
- FluoroFinder tracks 678 commercially available Vio Blue conjugates, searchable by target, clone, host and application.