Oxazole Blue
Oxazole Blue (Ex-Max 434 nm/Em-Max 457 nm)
Oxazole Blue (PO-PRO-1) is a cell-impermeant, fluorogenic intercalating nucleic acid stain. Oxazole Blue has an excitation peak at 434 nm and an emission peak at 457 nm. It is commonly used as a dead and apoptotic cell stain in the following applications: Fluorescence Microscopy and Flow Cytometry.
Excitation & emission spectra
Key facts
- Excitation maximum
- 434 nm
- Emission maximum
- 457 nm
- Optimal laser line
- 405
- Optimal filter
- 450/50
Oxazole Blue has an excitation maximum at 434 nm and an emission maximum at 457 nm, a Stokes shift of 23 nm. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is Oxazole Blue?
- Oxazole Blue (PO-PRO-1) is a cell-impermeant, fluorogenic intercalating nucleic acid stain. Oxazole Blue has an excitation peak at 434 nm and an emission peak at 457 nm. It is commonly used as a dead and apoptotic cell stain in the following applications: Fluorescence Microscopy and Flow Cytometry.
- What laser excites Oxazole Blue?
- Oxazole Blue is optimally excited by the 405 laser line. Its excitation maximum is 434 nm.
- What filter should I use to detect Oxazole Blue?
- A 450/50 filter captures the peak of the Oxazole Blue emission near 457 nm with minimal spillover.