355

Laser Line

450/50

Common Filter

354

Excitation Max

456

Emission Max

10

Antibodies


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About DAPI


4′,6-diamidino-2-phenylindole (DAPI) is a blue-emitting fluorophore that can be excited by the 355 nm blue laser and captured with a 450/50 nm bandpass filter. DAPI has an excitation peak at 350 nm and an emission peak at 465 nm, and is spectrally similar to Hoechst (33342), AlexaFluor® 350 or DyLight™ 350 DAPI is a commonly used DNA stain that binds to AT regions and has many favorable properties. DAPI's fluorescence increases upon binding to DNA, which is beneficial for signal to background ratios. Additionally, DAPI passes through both and live cell membranes, with varied success. It passes through dead cell membranes much more effectively making it better for fixed, dead cell staining. It can be used with live cells, but this requires a higher concentration of the dye. The sum of DAPI’s favorable properties make it a favorite dye for many applications under the umbrella of fluorescent microscopy, such as cell/nuclear quantitation, as an apoptosis detector or membrane viability dye, among others. DAPI is a generic dye sold by many suppliers.


Popular DAPI Antibodies


Company/SKU Applications Marker / Reactivity Host / Isotype / Clone Size / Price Details
Thermo Fisher Scientific
D3571
FC DAPI
All Species
N/A
IgG
Polyclonal
10 mg
$89
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BioLegend
422801
ICS,ICC DAPI
All Species
N/A
IgG
10 mg
$94
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Beckman Coulter
B30437
See TDS DAPI
All Species
N/A
N/A
N/A
200 Tests
$205
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Enzo Life Sciences
ENZ-52404
FC DAPI
All Species
N/A
N/A
N/A
100 mg
$535
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BD Biosciences
564907
IF,FC DAPI
All Species
N/A
N/A
N/A
1 mg
$49
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Bio-Rad
1351303
IF,FC DAPI
All Species
N/A
IgG
250 μg
$116
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Miltenyi Biotec
130-111-570
IF,FC DAPI
All Species
N/A
IgG
1 ml
$147
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Novus Biologicals
NBP2-31156-1mg
FC,IHC,ICC, IF,IHC-P,IHC-Fr,IHC-Free,IHC-WM,IF,ICC DAPI
N/A
IgG
1 mg
$99
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