About DAPI

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DAPI (4',6-diamidino-2-phenylindole (DAPI) is a blue-emitting fluorogenic DNA-binding dye that can be excited by the 355 nm blue laser and captured with a 450/50 nm bandpass filter. DAPI has an excitation peak at 350 nm and an emission peak at 465 nm. DAPI is a commonly used DNA stain that binds to AT regions and has many favorable properties. DAPI's fluorescence increases upon binding to DNA, which is beneficial for signal to background ratios. Additionally, DAPI passes through both dead and live cell membranes, with varied success. It passes through dead cell membranes much more effectively making it better for fixed, dead cell staining. It can be used with live cells, but this requires a higher concentration of the dye or can be used to detect membrane depolarization in apoptosis. DAPI is a generic dye sold by many suppliers.

Dyes similar to DAPI based on emission

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Calcein Violet AM CellTrace Violet CellVue Lavender CF350 CF405M CF408 Atlantic Blue DiFMU pH3.0 DiFMU pH9.0 DY-360XL eFluor 450 eFluor 455UV Fix Viability ExoBrite 410/450 EV Membrane Stain FxCycle Violet Ghost Dye Violet 450 Hoechst (33258) iFluor 350 Live/Dead Fix Violet Live-or-Dye 350/448 Live-or-Dye 405/452 LysoView 405 Marina Blue NADH Nuclear Blue Nuclear-ID Blue Nuclear Violet Oxazole Blue Oxazole Blue Homodimer Pacific Blue PhenoVue Hoechst 33342 Nuclear Stain POPO-1 ReadiLink 405-454 TagBFP Tag-It Violet V450 ViaFluor 405 Viobility 405/452 Fixable Dye VioBlue violetFluor 450 VivaFix 410/450 Zombie UV