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Tag-It Violet

Tag-It Violet (Ex-Max 390 nm/Em-Max 455 nm)

Tag-It Violet Cell Proliferation kit from Biolegend is a cell-permeant probe used for long-term cell tracking and proliferation studies with an excitation peak at 395 nm and an emission peak at 455 nm. It is commonly used in flow cytometry and fluorescence microscopy.

Excitation & emission spectra

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Key facts

Excitation maximum
395 nm
Emission maximum
455 nm
Optimal laser line
405
Optimal filter
450/50

Tag-It Violet has an excitation maximum at 395 nm and an emission maximum at 455 nm, a Stokes shift of 60 nm. It is optimally excited by the 405 laser line.

Frequently asked questions

What is Tag-It Violet?
Tag-It Violet Cell Proliferation kit from Biolegend is a cell-permeant probe used for long-term cell tracking and proliferation studies with an excitation peak at 395 nm and an emission peak at 455 nm. It is commonly used in flow cytometry and fluorescence microscopy.
What laser excites Tag-It Violet?
Tag-It Violet is optimally excited by the 405 laser line. Its excitation maximum is 395 nm.
What filter should I use to detect Tag-It Violet?
A 450/50 filter captures the peak of the Tag-It Violet emission near 455 nm with minimal spillover.