eFluor 450
eFluor™ 450 (Ex-Max 405 nm/Em-Max 450 nm)
eFluor™ 450 (eF450) from Thermo Fisher Scientific has an excitation peak at 405 nm and an emission peak at 450 nm. It is useful in both flow cytometry and fluorescence microscopy.
Excitation & emission spectra
Key facts
- Excitation maximum
- 405 nm
- Emission maximum
- 450 nm
- Optimal laser line
- 405
- Optimal filter
- 450/50
- Relative brightness
- 2 of 5
- Commercial conjugates
- 384 from 3 suppliers
eFluor 450 has an excitation maximum at 405 nm and an emission maximum at 450 nm, a Stokes shift of 45 nm. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is eFluor 450?
- eFluor™ 450 (eF450) from Thermo Fisher Scientific has an excitation peak at 405 nm and an emission peak at 450 nm. It is useful in both flow cytometry and fluorescence microscopy.
- What laser excites eFluor 450?
- eFluor 450 is optimally excited by the 405 laser line. Its excitation maximum is 405 nm.
- What filter should I use to detect eFluor 450?
- A 450/50 filter captures the peak of the eFluor 450 emission near 450 nm with minimal spillover.
- How bright is eFluor 450?
- eFluor 450 has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to eFluor 450?
- FluoroFinder tracks 384 commercially available eFluor 450 conjugates, searchable by target, clone, host and application.