About Alexa Fluor 555

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Alexa Fluor® 555 (AF555, Alexa 555) is a yellow-emitting synthetic fluorophore that can be excited by the 532 nm yellow laser and captured with a 575/36 nm bandpass filter. AF555 has an excitation peak at 555 nm and an emission peak at 580 nm, and is spectrally similar to Cy®3 (GE Healthcare), TRITC, iFluor™ 555 (ATT Bioquest) and iFluor™ 560 (ATT Bioquest), and Pacific Orange (ThermoFisher Scientific). It is uncommon for flow cytometers to be equipped with 532 lasers, so while AF555 can be used for flow cytometry, it is less popular for this application. This dye is commonly used for immunohistochemistry applications and more specifically super-resolution microscopy because it is bright, moderately photostable, and pH insensitive. The Alexa Fluor dye family was originally developed by Molecular Probes, now a part of ThermoFisher Scientific. AlexaFluor is a registered trademark of Invitrogen, ThermoFisher Scientific. Alexa Fluor dyes come in a wide variety of modified formats that allow for easy conjugations or right out of the box use.

Dyes similar to Alexa Fluor 555 based on emission

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10-Acetyl-37-dihydroxyphenoxazin 5-TAMRA 6-TAMRA alamarBlue Alexa Fluor 546 Alizarin Red Amplex Red ATTO 550 BPE Calcein Red AM Calcein Red Calcein Red-Orange AM Calcium Orange CellBrite Steady 550 CellMask Orange Plasma Membrane Stain CellTrace Yellow CF550R CF568 CF568ST CMA-Chromomycin A3 CytoTrace Red DsRed2 dsRed DsRed Express dTomato DY-510XL DyLight 549 DyLight 550 Dyomics 547 eFluor 585NC ExoBrite 560/585 EV Membrane Stain FVS570 Janelia Fluor 549 Kaede (red) LipidSpot 488 Live-or-Dye 568/583 MagDot 575 MaxLight 550 MemBrite Fix 568/580 MITO-ID MP (high potential) MitoTracker Orange mScarlet mTangerine MultiDot 575 NeuroVue Red Nile Red NL 557 NovaFluor Blue 585 NovaFluor Yellow 590 PE PhenoVue 512 Nucleic Acid Stain PhenoVue 551 Mitochondrial Stain PhenoVue 577 Lysosomal Stain pHrodo Red PromoFluor 555P Qdot 585 Resazurin Resorufin RFP Rhod-2 (Ca2+ bound) Rhod-5N (Ca2+ bound) Rhodamine RY586 Seta-400-NHS SNARF 1 pH6.0 Spark YG 581 SureLight BPE SureLight RPE Synapto Green TagRFP tdTomato TMRE TRITC VivaFix 547/573 VRDye 549