532

Laser Line

575/36

Common Filter

555

Excitation Max

580

Emission Max

7,221

Antibodies

1

Conjugation Kits

3

Services


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About Alexa Fluor 555


Alexa Fluor® 555 (AF555, Alexa 555) is a yellow-emitting synthetic fluorophore that can be excited by the 532 nm yellow laser and captured with a 575/36 nm bandpass filter. AF555 has an excitation peak at 555 nm and an emission peak at 580 nm, and is spectrally similar to Cy®3 (GE Healthcare), TRITC, iFluor™ 555 (ATT Bioquest) and iFluor™ 560 (ATT Bioquest), and Pacific Orange (ThermoFisher Scientific). It is uncommon for flow cytometers to be equipped with 532 lasers, so while AF555 can be used for flow cytometry, it is less popular for this application. This dye is commonly used for immunohistochemistry applications and more specifically super-resolution microscopy because it is bright, moderately photostable, and pH insensitive. The Alexa Fluor dye family was originally developed by Molecular Probes, now a part of ThermoFisher Scientific. AlexaFluor is a registered trademark of Invitrogen, ThermoFisher Scientific. Alexa Fluor dyes come in a wide variety of modified formats that allow for easy conjugations or right out of the box use.


Popular Alexa Fluor 555 Antibodies


Company/SKU Applications Marker / Reactivity Host / Isotype / Clone Size / Price Details
BD Biosciences
558617
Bioimaging Ki-67
Mouse,Human,Rat,Rhesus
Mouse
IgG1 κ
B56
100 Tests
$415
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Bioss
bs-6477R-A555
CD95
Human,Mouse,Rat
Rabbit
IgG
100 μl
$470
View
Bioss
bs-1014R-A555
FC CD11b/c
Human,Mouse,Rat
Rabbit
IgG
100 μl
$470
View
Bioss
bs-0672R-A555
IHC-P CD117
Human,Mouse,Rat,Canine
Rabbit
IgG
100 μl
$470
View
Bioss
bs-10009R-A555
FC,IF,IHC CD324
Human,Mouse,Rat
Rabbit
IgG
100 μl
$470
View
Bioss
bs-2370R-A555
FABP5
Human,Mouse,Rat
Rabbit
IgG
100 μl
$457
View
Bioss
bs-0938R-A555
FC CD314
Human,Mouse,Rat
Rabbit
IgG
100 μl
$470
View
Cell Signaling Technology
39680S
IF,FC Neutrophil Elastase
Human
Rabbit
IgG
100 μl
$358
View
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