BODIPY 630/650
BODIPY 630/650 (Ex-Max 623 nm/Em-Max 639 nm)
BODIPY 630/650 is used as a lipophilic probe for polar and neutral lipids typically used to detect oil and nonpolar lipid droplets in live or fixed cells. It has an excitation peak at 630 nm and an emission peak at 650 nm. The succinimidyl ester form of this dye can be used to conjugate hydrophobic proteins or lipids.
Excitation & emission spectra
Key facts
- Excitation maximum
- 623 nm
- Emission maximum
- 639 nm
- Optimal laser line
- 628-640
- Optimal filter
- 630/69
- Molecular weight
- 660.5
BODIPY 630/650 has an excitation maximum at 623 nm and an emission maximum at 639 nm, a Stokes shift of 16 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is BODIPY 630/650?
- BODIPY 630/650 is used as a lipophilic probe for polar and neutral lipids typically used to detect oil and nonpolar lipid droplets in live or fixed cells. It has an excitation peak at 630 nm and an emission peak at 650 nm. The succinimidyl ester form of this dye can be used to conjugate hydrophobic proteins or lipids.
- What laser excites BODIPY 630/650?
- BODIPY 630/650 is optimally excited by the 628-640 laser line. Its excitation maximum is 623 nm.
- What filter should I use to detect BODIPY 630/650?
- A 630/69 filter captures the peak of the BODIPY 630/650 emission near 639 nm with minimal spillover.