About PE-Fire 640

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PE-Fire™ 640 from Biolegend is a red-emitting tandem fluorophore that combines pycoerythrin (PE) and Fire™ 640. The donor molecule, PE can be excited by the 488-nm blue, 532-nm green, or 561-nm yellow-green laser and and transfers energy to the acceptor molecule, Fire™ 640, which emitts light that can be captured with a 630/69 nm bandpass filter. PE-eF610 has an excitation peak at 565 nm and an emission peak at 639 nm. In spectral cytometry, this dye can be accurately unmixed from other dyes with highly overlapping spectral profiles, such as PE-Dazzle® 594 and PE-Cy5. PE-Fire™ 640 is recommended for labeling antigens that: (1) are expressed on a small subset (2) will be excluded from final analysis, and (3) require a bright signal but are not co-expressed with an antigen marked by a dye with overlapping spectra.

Alternative Dyes to PE-Fire 640

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PE-Alexa 610

Dyes similar to PE-Fire 640 based on emission

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7-AAD Alexa Fluor 633 Alexa Fluor 635 ATTO 610 ATTO Rho14 Brilliant Blue 630-P2 BDY 630-X SE BODIPY 630/650 Brilliant Violet 650 CF620R CytoTrack Red DY-480XL DY-601XL E2-Crimson Katushka2S LIVE 610 LysoView 540 LysoView 594 MagDot 640 MITO-ID (Extracellular O2) MitoView 633 mKate2 mKate mNeptune mPlum MultiDot 640 Nile Red Oxazole Red Homodimer PhenoVue Nile Red Lipid Stain SNARF 1 pH9.0 STAR 520L SuperBright 645 SureLight RPC Syto 61 Sytox AADvanced YO-PRO-3 YOYO-3