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mNeptune

mNeptune (Ex-Max 598 nm/Em-Max 643 nm)

<html>mNeptune is a monomeric red fluorescent protein with excitation and emission maximums of 600 nm and 650 nm, respectively. mNeptune was first derived from <i>Entacmaea quadricolor.</i></html>

Excitation & emission spectra

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Key facts

Excitation maximum
598 nm
Emission maximum
643 nm
Optimal laser line
594
Optimal filter
630/69
Extinction coefficient
67000
Quantum yield
0.2

mNeptune has an excitation maximum at 598 nm and an emission maximum at 643 nm, a Stokes shift of 45 nm. Its extinction coefficient is 67000 M⁻¹cm⁻¹. It is optimally excited by the 594 laser line.

Frequently asked questions

What is mNeptune?
<html>mNeptune is a monomeric red fluorescent protein with excitation and emission maximums of 600 nm and 650 nm, respectively. mNeptune was first derived from <i>Entacmaea quadricolor.</i></html>
What laser excites mNeptune?
mNeptune is optimally excited by the 594 laser line. Its excitation maximum is 598 nm.
What filter should I use to detect mNeptune?
A 630/69 filter captures the peak of the mNeptune emission near 643 nm with minimal spillover.