CytoTrack Red
CytoTrack Red (Ex-Max 624 nm/Em-Max 640 nm)
CytoTrack Red from Bio-Rad passively crosses the cell membrane and is hydrolyzed causing it to become fluorescent and retained intracellularly. It then covalently crosslinks with primary amine-containing proteins inside the cell. With each daughter generation, as the cell divides, the intensity of the dye will become half of it's parent, indicating a symmetrical division of the cell and cytoplasm. CytoTrack Red has an excitation peak at 624 nm and an emission peak at 640 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 624 nm
- Emission maximum
- 640 nm
- Optimal laser line
- 628-640
- Optimal filter
- 630/69
CytoTrack Red has an excitation maximum at 624 nm and an emission maximum at 640 nm, a Stokes shift of 16 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is CytoTrack Red?
- CytoTrack Red from Bio-Rad passively crosses the cell membrane and is hydrolyzed causing it to become fluorescent and retained intracellularly. It then covalently crosslinks with primary amine-containing proteins inside the cell. With each daughter generation, as the cell divides, the intensity of the dye will become half of it's parent, indicating a symmetrical division of the cell and cytoplasm. CytoTrack Red has an excitation peak at 624 nm and an emission peak at 640 nm.
- What laser excites CytoTrack Red?
- CytoTrack Red is optimally excited by the 628-640 laser line. Its excitation maximum is 624 nm.
- What filter should I use to detect CytoTrack Red?
- A 630/69 filter captures the peak of the CytoTrack Red emission near 640 nm with minimal spillover.