Synapto Green
Synapto Green (Ex-Max 473 nm/Em-Max 579 nm)
Synapto Green (FM1-43) from Biotium is a lipophilic dye that intercalates into the cellular membrane and is often used to monitor endocytictic vesicles and membrane recyling. It has an excitation peak at 505 nm and an emission peak at 598 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 473 nm
- Emission maximum
- 598 nm
- Optimal laser line
- 488
- Optimal filter
- 585/42
- Molecular weight
- 608
Synapto Green has an excitation maximum at 473 nm and an emission maximum at 598 nm, a Stokes shift of 125 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Synapto Green?
- Synapto Green (FM1-43) from Biotium is a lipophilic dye that intercalates into the cellular membrane and is often used to monitor endocytictic vesicles and membrane recyling. It has an excitation peak at 505 nm and an emission peak at 598 nm.
- What laser excites Synapto Green?
- Synapto Green is optimally excited by the 488 laser line. Its excitation maximum is 473 nm.
- What filter should I use to detect Synapto Green?
- A 585/42 filter captures the peak of the Synapto Green emission near 598 nm with minimal spillover.