LysoBrite Red
LysoBrite Red (Ex-Max 575 nm/Em-Max 596 nm)
LysoBrite Red from AAT Bioquest is a cell-permeant alkaline probe that localizes to acidic compartments inside the cell, such as endosomes and lysosomes. These types of probes are designed for live-cell imaging and do not typically survive fixation and permeabilization. LysoBrite Red has an excitation peak at 575 nm and an emission peak at 596 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 575 nm
- Emission maximum
- 596 nm
- Optimal laser line
- 561
- Optimal filter
- 585/40
LysoBrite Red has an excitation maximum at 575 nm and an emission maximum at 596 nm, a Stokes shift of 21 nm. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is LysoBrite Red?
- LysoBrite Red from AAT Bioquest is a cell-permeant alkaline probe that localizes to acidic compartments inside the cell, such as endosomes and lysosomes. These types of probes are designed for live-cell imaging and do not typically survive fixation and permeabilization. LysoBrite Red has an excitation peak at 575 nm and an emission peak at 596 nm.
- What laser excites LysoBrite Red?
- LysoBrite Red is optimally excited by the 561 laser line. Its excitation maximum is 575 nm.
- What filter should I use to detect LysoBrite Red?
- A 585/40 filter captures the peak of the LysoBrite Red emission near 596 nm with minimal spillover.