Alexa Fluor 532
Alexa Fluor™ 532 (Ex-Max 532 nm/Em-Max 554 nm)
Alexa Fluor™ 532 (AF532, Alexa 532) has an excitation peak at 532 nm and an emission peak at 554 nm, and is spectrally similar to Cy®3 (GE Healthcare), TRITC, iFluor® 514 (ATT Bioquest) and iFluor® 546 (ATT Bioquest). Many flow cytometers are equipped with 532nm lasers, so while Alexa 532 can be used for flow cytometry, PE is more commonly used due to its brightness and spectral characteristics. This dye is commonly used for fluorescence microscopy applications.
Excitation & emission spectra
Key facts
- Excitation maximum
- 532 nm
- Emission maximum
- 554 nm
- Optimal laser line
- 532
- Optimal filter
- 575/36
- Relative brightness
- 1 of 5
- Extinction coefficient
- 81000
- Quantum yield
- 0.61
- Molecular weight
- 627
- Commercial antibodies
- 23.2k from 8 suppliers
Alexa Fluor 532 has an excitation maximum at 532 nm and an emission maximum at 554 nm, a Stokes shift of 22 nm. Its extinction coefficient is 81000 M⁻¹cm⁻¹. It is optimally excited by the 532 laser line.
Frequently asked questions
- What is Alexa Fluor 532?
- Alexa Fluor™ 532 (AF532, Alexa 532) has an excitation peak at 532 nm and an emission peak at 554 nm, and is spectrally similar to Cy®3 (GE Healthcare), TRITC, iFluor® 514 (ATT Bioquest) and iFluor® 546 (ATT Bioquest). Many flow cytometers are equipped with 532nm lasers, so while Alexa 532 can be used for flow cytometry, PE is more commonly used due to its brightness and spectral characteristics. This dye is commonly used for fluorescence microscopy applications.
- What laser excites Alexa Fluor 532?
- Alexa Fluor 532 is optimally excited by the 532 laser line. Its excitation maximum is 532 nm.
- What filter should I use to detect Alexa Fluor 532?
- A 575/36 filter captures the peak of the Alexa Fluor 532 emission near 554 nm with minimal spillover.
- How bright is Alexa Fluor 532?
- Alexa Fluor 532 has a relative brightness of 1 out of 5 (quantum yield 0.61).
- How many antibodies are available conjugated to Alexa Fluor 532?
- FluoroFinder tracks 23197 commercially available Alexa Fluor 532 conjugates, searchable by target, clone, host and application.