What is LYSO-ID Green?

LYSO-ID Green from Enzo Life Sciences is a cell-permeant alkaline probe that localizes to acidic compartments inside the cell, such as endosomes and lysosomes. These types of probes are designed for live-cell imaging and do not typically survive fixation and permeabilization. LYSO-ID Green has an excitation peak at 480 nm and an emission peak at 545 nm.

What laser excites LYSO-ID Green?

LYSO-ID Green is optimally excited by the 488 laser line. Its excitation maximum is 480 nm.

What filter should I use to detect LYSO-ID Green?

A 550/40 filter captures the peak of the LYSO-ID Green emission near 545 nm with minimal spillover.

LYSO-ID Green Dye Profile

Excitation & emission spectra

Key facts

Excitation maximum: 480 nm
Emission maximum: 545 nm
Optimal laser line: 488
Optimal filter: 550/40

LYSO-ID Green has an excitation maximum at 480 nm and an emission maximum at 545 nm, a Stokes shift of 65 nm. It is optimally excited by the 488 laser line.

Frequently asked questions

What is LYSO-ID Green?: LYSO-ID Green from Enzo Life Sciences is a cell-permeant alkaline probe that localizes to acidic compartments inside the cell, such as endosomes and lysosomes. These types of probes are designed for live-cell imaging and do not typically survive fixation and permeabilization. LYSO-ID Green has an excitation peak at 480 nm and an emission peak at 545 nm.
What laser excites LYSO-ID Green?: LYSO-ID Green is optimally excited by the 488 laser line. Its excitation maximum is 480 nm.
What filter should I use to detect LYSO-ID Green?: A 550/40 filter captures the peak of the LYSO-ID Green emission near 545 nm with minimal spillover.