CF517ST
CF®517ST (Ex-Max 516 nm/Em-Max 548 nm)
CF®517ST from Biotium is a fluorophore with an excitation peak at 516 nm and an emission peak at 548 nm. Due to its unique spectral characteristics, it could be useful in spectral flow cytometry.
Excitation & emission spectra
Key facts
- Excitation maximum
- 516 nm
- Emission maximum
- 548 nm
- Optimal laser line
- 488
- Optimal filter
- 530/43
- Relative brightness
- 2 of 5
CF517ST has an excitation maximum at 516 nm and an emission maximum at 548 nm, a Stokes shift of 32 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is CF517ST?
- CF®517ST from Biotium is a fluorophore with an excitation peak at 516 nm and an emission peak at 548 nm. Due to its unique spectral characteristics, it could be useful in spectral flow cytometry.
- What laser excites CF517ST?
- CF517ST is optimally excited by the 488 laser line. Its excitation maximum is 516 nm.
- What filter should I use to detect CF517ST?
- A 530/43 filter captures the peak of the CF517ST emission near 548 nm with minimal spillover.
- How bright is CF517ST?
- CF517ST has a relative brightness of 2 out of 5.