CF405L
CF®405L (Ex-Max 395 nm/Em-Max 545 nm)
CF®405L from Biotium is a fluorophore with an excitation peak at 395 nm and an emission peak at 545 nm. It is spectrally similar to Pacific Orange, Cascade Yellow and Krome Orange and can be used in flow cytometry. A superior alternative in flow cytometry is StarBright 570 and Brilliant™ Violet 510.
Excitation & emission spectra
Key facts
- Excitation maximum
- 395 nm
- Emission maximum
- 545 nm
- Optimal laser line
- 405
- Optimal filter
- 530/43
- Relative brightness
- 1 of 5
- Extinction coefficient
- 24000
- Molecular weight
- 1573
- Commercial antibodies
- 28 from 1 supplier
CF405L has an excitation maximum at 395 nm and an emission maximum at 545 nm, a Stokes shift of 150 nm. Its extinction coefficient is 24000 M⁻¹cm⁻¹. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is CF405L?
- CF®405L from Biotium is a fluorophore with an excitation peak at 395 nm and an emission peak at 545 nm. It is spectrally similar to Pacific Orange, Cascade Yellow and Krome Orange and can be used in flow cytometry. A superior alternative in flow cytometry is StarBright 570 and Brilliant™ Violet 510.
- What laser excites CF405L?
- CF405L is optimally excited by the 405 laser line. Its excitation maximum is 395 nm.
- What filter should I use to detect CF405L?
- A 530/43 filter captures the peak of the CF405L emission near 545 nm with minimal spillover.
- How bright is CF405L?
- CF405L has a relative brightness of 1 out of 5.
- How many antibodies are available conjugated to CF405L?
- FluoroFinder tracks 28 commercially available CF405L conjugates, searchable by target, clone, host and application.