Dyes similar to NucSpot 470 based on emissionAlexa Fluor 430 Alexa Fluor 514 Alexa Fluor 532 ATTO 532 BODIPY R6G Calcein Orange Cascade Yellow CF405L CF450 CF514 CF517ST CPN550 Yellow DY-375XL ER-ID Green Ghost Dye Violet 540 iFluor 514 Janelia Fluor 525 Live/Dead Fix Yellow Live-or-Dye 405/545 Live-or-Dye 510/550 LysoBrite Orange LYSO-ID Green LysoSensor Yellow/Blue pH3.0 mBanana mHoneyDew MitoLite Orange EX405 NBD NovaFluor Blue 555 Nuclear Orange Oxazole Gold Pacific Orange Qdot 545 ReadiLink 405-537 Rhodamine 6G Spark Blue 550 Spark Violet 538 VivaFix 398/550
About NucSpot 470
Biotium’s NucSpot 470 is a cell membrane-impermeant dye that is fluorescent upon binding DNA. Since it is cell membrane-impermeant, it can be used to counterstain nuclei of fixed cells, or stain nuclei in dead cells. Other similar DNA binding dyes will often stain the nucleus and cytoplasm, while NucSpot 470 is specific to the nucleus, decreasing background signal. The more general NucSpot family of dyes has a few more members that have different functions compared to NucSpot 470. NucSpot Far-Red is an alternative to 7-AAD and is similarly used for staining cells with compromised cellular membranes, while NucSpot Live dyes are cell membrane-permeant DNA stains used for live cell staining. Check out the other members of this dye line in FluoroFinder’s Dye Directory or on our Spectra Viewer.