NucSpot 470
NucSpot 470 (Ex-Max 460 nm/Em-Max 546 nm)
Biotium's NucSpot 470 is a cell membrane-impermeant dye that is fluorescent upon binding DNA. Since it is cell membrane-impermeant, it can be used to counterstain nuclei of fixed cells, or stain nuclei in dead cells. Other similar DNA binding dyes will often stain the nucleus and cytoplasm, while NucSpot 470 is specific to the nucleus, decreasing background signal. The more general NucSpot family of dyes has a few more members that have different functions compared to NucSpot 470. NucSpot Far-Red is an alternative to 7-AAD and is similarly used for staining cells with compromised cellular membranes, while NucSpot Live dyes are cell membrane-permeant DNA stains used for live cell staining. Check out the other members of this dye line in FluoroFinder's Dye Directory or on our Spectra Viewer.
Excitation & emission spectra
Key facts
- Excitation maximum
- 460 nm
- Emission maximum
- 546 nm
- Optimal laser line
- 488
- Optimal filter
- 525/50
NucSpot 470 has an excitation maximum at 460 nm and an emission maximum at 546 nm, a Stokes shift of 86 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is NucSpot 470?
- Biotium's NucSpot 470 is a cell membrane-impermeant dye that is fluorescent upon binding DNA. Since it is cell membrane-impermeant, it can be used to counterstain nuclei of fixed cells, or stain nuclei in dead cells. Other similar DNA binding dyes will often stain the nucleus and cytoplasm, while NucSpot 470 is specific to the nucleus, decreasing background signal. The more general NucSpot family of dyes has a few more members that have different functions compared to NucSpot 470. NucSpot Far-Red is an alternative to 7-AAD and is similarly used for staining cells with compromised cellular membranes, while NucSpot Live dyes are cell membrane-permeant DNA stains used for live cell staining. Check out the other members of this dye line in FluoroFinder's Dye Directory or on our Spectra Viewer.
- What laser excites NucSpot 470?
- NucSpot 470 is optimally excited by the 488 laser line. Its excitation maximum is 460 nm.
- What filter should I use to detect NucSpot 470?
- A 525/50 filter captures the peak of the NucSpot 470 emission near 546 nm with minimal spillover.