About Live/Dead Fix Yellow

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The Live/Dead Fixable Viability probes from Thermo Fisher are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live/Dead Fixable Yellow has an excitation peak at 400 nm and an emission peak at 550 nm.

Alternative Dyes to Live/Dead Fix Yellow

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FVS575V Ghost Dye Violet 540 VivaFix 398/550

Dyes similar to Live/Dead Fix Yellow based on emission

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Alexa Fluor 430 Alexa Fluor 532 ATTO 532 BODIPY R6G Calcein Orange Cascade Yellow CF405L CF514 CF517ST CF532 CoraFluor-1 CoraFluor-2 CPN550 Yellow CytoTrack Yellow DY-375XL EFLUXX-ID Gold ER-ID Green Ghost Dye Violet 540 iFluor 514 iFluor 532 Janelia Fluor 525 Live-or-Dye 405/545 Live-or-Dye 510/550 LysoBrite Orange LYSO-ID Green mBanana mHoneyDew MitoLite Orange EX405 NovaFluor Blue 555 Nuclear Orange NucSpot 470 Pacific Orange PhenoVue 512 Nucleic Acid Stain – complexed with nucleic acids Qdot 545 RealBlue 545 Rhodamine 6G VivaFix 398/550 Vybrant DyeCycle Orange XFD514 XFD532 cFluor V547 5(6)-Carboxyrhodamine cFluor B548 RayBright LIVE V500 ExoBrite 515/540 True EV Membrane Stain Lysosome Probe Orange CoraFluor 2, amine reactive