Calcein Orange
Calcein Orange (Ex-Max 523 nm/Em-Max 549 nm)
Calcein Orange is the hydrodlyzed version of Calcein Orange, AM. It is commonly used as a cell impermeant, esterase independet control for Calcein Orange, AM. Commonly used in fluorescence microscopy, flow cytometry, or fluorescent microplate reader assays. It has an excitation peak at 523 nm and an emission peak at 549 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 523 nm
- Emission maximum
- 549 nm
- Optimal laser line
- 532
- Optimal filter
- 525/50
Calcein Orange has an excitation maximum at 523 nm and an emission maximum at 549 nm, a Stokes shift of 26 nm. It is optimally excited by the 532 laser line.
Frequently asked questions
- What is Calcein Orange?
- Calcein Orange is the hydrodlyzed version of Calcein Orange, AM. It is commonly used as a cell impermeant, esterase independet control for Calcein Orange, AM. Commonly used in fluorescence microscopy, flow cytometry, or fluorescent microplate reader assays. It has an excitation peak at 523 nm and an emission peak at 549 nm.
- What laser excites Calcein Orange?
- Calcein Orange is optimally excited by the 532 laser line. Its excitation maximum is 523 nm.
- What filter should I use to detect Calcein Orange?
- A 525/50 filter captures the peak of the Calcein Orange emission near 549 nm with minimal spillover.