About Live-or-Dye 405/545

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Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 405/545 has an excitation peak at 405 nm and an emission peak at 545 nm.

Alternative Dyes to Live-or-Dye 405/545

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FVS575V Ghost Dye Violet 540 VivaFix 398/550

Dyes similar to Live-or-Dye 405/545 based on emission

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2-NBDG Alexa Fluor 430 Alexa Fluor 514 Alexa Fluor 532 ATTO 532 BODIPY R6G Calcein Orange Cascade Yellow CF405L CF450 CF514 CF517ST CoraFluor-1 CoraFluor-2 CPN550 Yellow DY-375XL ER-ID Green Ghost Dye Violet 540 iFluor 514 Janelia Fluor 525 Live/Dead Fix Yellow Live-or-Dye 510/550 LysoBrite Orange LYSO-ID Green LysoSensor Yellow/Blue pH3.0 mBanana mHoneyDew MitoLite Orange EX405 NBD NovaFluor Blue 555 Nuclear Orange NucSpot 470 Oxazole Gold Pacific Orange PhenoVue 512 Nucleic Acid Stain – complexed with nucleic acids Qdot 545 RealBlue 545 Rhodamine 6G Spark Blue 550 Spark Violet 538 VivaFix 398/550 Vybrant DyeCycle Orange RayBright LIVE V500 ExoBrite 515/540 True EV Membrane Stain Lysosome Probe Orange CoraFluor 2, amine reactive Spark PLUS B550 cFluor V547 XFD514 XFD532 5(6)-Carboxyrhodamine cFluor B548