MiCy1
MiCy1 (Ex-Max 472 nm/Em-Max 495 nm)
MiCy1 (Midoriishi Cyan) is a dimeric fluorescent protein. It has an excitation peak at 472 nm and an emission peak at 495 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 472 nm
- Emission maximum
- 495 nm
- Optimal laser line
- 488
- Optimal filter
- 515/30
- Extinction coefficient
- 27250
- Quantum yield
- 0.9
MiCy1 has an excitation maximum at 472 nm and an emission maximum at 495 nm, a Stokes shift of 23 nm. Its extinction coefficient is 27250 M⁻¹cm⁻¹. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is MiCy1?
- MiCy1 (Midoriishi Cyan) is a dimeric fluorescent protein. It has an excitation peak at 472 nm and an emission peak at 495 nm.
- What laser excites MiCy1?
- MiCy1 is optimally excited by the 488 laser line. Its excitation maximum is 472 nm.
- What filter should I use to detect MiCy1?
- A 515/30 filter captures the peak of the MiCy1 emission near 495 nm with minimal spillover.