IgM Secondary Antibody / Pacific Blue / MHM-88
IgM Secondary Antibody / Pacific Blue / MHM-88
Citations:
(9)
Product Details
| Supplier | BioLegend | |
|---|---|---|
| Catalog #: | 314513 (View supplier product page) | |
| Size | 25 μg | |
| Price | $165.00 | |
| Antigen | IgM | |
| Clone | MHM-88 | |
| Host | Mouse | |
| Isotype | IgG1 κ | |
| Conjugate | Pacific Blue | |
| Target Species | Human | |
| Applications | FC | |
| Description | Pacific Blue anti-human IgM |
About IgM and Pacific Blue
| IgM | Immunoglobulins (Ig) are the antigen recognition molecules of B cells. An Ig molecule is made up of 2 identical heavy chains and 2 identical light chains (see MIM 147200) joined by disulfide bonds so that each heavy chain is linked to a light chain and the 2 heavy chains are linked together. Each Ig heavy chain has an N-terminal variable (V) region containing the antigen-binding site and a C-terminal constant (C) region, encoded by an individual C region gene, that determines the isotype of the antibody and provides effector or signaling functions. The heavy chain V region is encoded by 1 each of 3 types of genes: V genes (see MIM 147070), joining (J) genes (see MIM 147010), and diversity (D) genes (see MIM 146910). The C region genes are clustered downstream of the V region genes within the heavy chain locus on chromosome 14. The IGHM gene encodes the C region of the mu heavy chain, which defines the IgM isotype. Naive B cells express the transmembrane forms of IgM and IgD (see IGHD; MIM 1471770) on their surface. During an antibody response, activated B cells can switch to the expression of individual downstream heavy chain C region genes by a process of somatic recombination known as isotype switching. In addition, secreted Ig forms that act as antibodies can be produced by alternative RNA processing of the heavy chain C region sequences. Although the membrane forms of all Ig isotypes are monomeric, secreted IgM forms pentamers, and occasionally hexamers, in plasma (summary by Janeway et al., 2005).[supplied by OMIM, Aug 2010] | |
|---|---|---|
| Pacific Blue | Pacific Blue® (Pac Blue, PacBlue) is a blue-emitting organic fluorophore that can be excited by the 405 nm Violet laser and captured with a 450/50 nm bandpass filter. Pacific Blue has an excititaion peak at 401 nm and an emission peak at 452 nm. |
Images
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Over night cultured human peripheral blood lymphocytes stained with CD19 (HIB19) PE and MHM-88 Pacific Blue™ (top) or mouse IgG1, κ Pacific Blue™ isotype control (bottom) |
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Citations
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Reviews & Ratings
Pacific Blue Excitation and Emission Spectra
$165.00
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