FxCycle Violet
FxCycle Violet (Ex-Max 358 nm/Em-Max 461 nm)
FxCycle Violet from Thermo Fisher Scientific is a fluorogenic cell-impermeant nucleic acid stain used for analysis of DNA content in fixed cells. It has an excitation peak at 358 nm and an emission peak at 461 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 358 nm
- Emission maximum
- 461 nm
- Optimal laser line
- 355/405
- Optimal filter
- 450/50
FxCycle Violet has an excitation maximum at 358 nm and an emission maximum at 461 nm, a Stokes shift of 103 nm. It is optimally excited by the 355/405 laser line.
Frequently asked questions
- What is FxCycle Violet?
- FxCycle Violet from Thermo Fisher Scientific is a fluorogenic cell-impermeant nucleic acid stain used for analysis of DNA content in fixed cells. It has an excitation peak at 358 nm and an emission peak at 461 nm.
- What laser excites FxCycle Violet?
- FxCycle Violet is optimally excited by the 355/405 laser line. Its excitation maximum is 358 nm.
- What filter should I use to detect FxCycle Violet?
- A 450/50 filter captures the peak of the FxCycle Violet emission near 461 nm with minimal spillover.