LysoView 540
LysoView™ 540 (Ex-Max 541 nm/Em-Max 634 nm)
Biotium's LysoView™ dyes are cell-permeant alkaline probes that localize to acidic compartments inside the cell, such as endosomes and lysosomes. These types of probes are designed for live-cell imaging and do not typically survive fixation and permeabilization. LysoView™ 540 has an excitation peak at 541 nm and an emission peak at 634 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 541 nm
- Emission maximum
- 634 nm
- Optimal laser line
- 532
- Optimal filter
- 630/69
LysoView 540 has an excitation maximum at 541 nm and an emission maximum at 634 nm, a Stokes shift of 93 nm. It is optimally excited by the 532 laser line.
Frequently asked questions
- What is LysoView 540?
- Biotium's LysoView™ dyes are cell-permeant alkaline probes that localize to acidic compartments inside the cell, such as endosomes and lysosomes. These types of probes are designed for live-cell imaging and do not typically survive fixation and permeabilization. LysoView™ 540 has an excitation peak at 541 nm and an emission peak at 634 nm.
- What laser excites LysoView 540?
- LysoView 540 is optimally excited by the 532 laser line. Its excitation maximum is 541 nm.
- What filter should I use to detect LysoView 540?
- A 630/69 filter captures the peak of the LysoView 540 emission near 634 nm with minimal spillover.